水華魚腥藻的超弱發(fā)光研究及甲磺隆抑制水華魚腥藻生長(zhǎng)的作用機(jī)理初探.pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文水華魚腥藻的超弱發(fā)光研究及甲磺隆抑制水華魚腥藻生長(zhǎng)的作用機(jī)理初探姓名：張新萍申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：農(nóng)藥學(xué)指導(dǎo)教師：董元彥20030501華中農(nóng)業(yè)大學(xué)2003年砸十學(xué)位論文ABSTRACTUseingBPCLultraweakchemiluminescenceanalyziertheultraweakbioluminescenceanddelayedbioluminescentspectrumofanabaena

2、flos—aquaehavebeenfirstsystematicallydetectedThecharacteristicandmeasurementconditionofultraweakbioluminescenceofanabaenaflosaquaearediscussedFurthermore，thechangeofultraweakbioluminescenceanddelayedbioluminescentspectru

3、mofanabaenaflosaquaeaftertheadditionofmetsulfuron—methylarestudiedAtthesametime，themechanismofmetsulfuron—methylonanabaenaflosaquaehasbeenpreliminarilystudiedfromthesensitivitiesofacetolactatesynthase(EC4，1，3，18，ALS)onme

4、tsulfuron—methylinvitroandinvivo，respectivelyTheresultsareshownasfollows：1Fromthestudyofultraweakluminescenceofanabaenaflos—aquae，revealededthat：1)thedetectionofultraweakbioluminescenceofanabaenaflos—aquaewithBPCLultrawe

5、akchemiluminescenceanalyzierfirstconfirmedblue—greenalgae，thelinkerbetweenbacteriaandalgae，havetheabilityofukraweakbioluminescence，whichshowedthatthespecieswithdifferentevolutionlevelscommonlyhavetheabilityofultraweakbio

6、luminescence；2)themeasuredphotocountrate—timecurveofultraweakluminescentfromanabaenaflos—aquaefollowsahyperbolicdecaylaw，indicatingthecoherenceofbiophotonemission3)Theintensionofultraweakbioluminescenceweredependentonthe

7、differentdevelopmentstagesDuringlogarithmicgrowthstagesultraweakbioluminescenceofAnabaenaflos—aquaewasstronglycorrelatedtotheconcentrationofAnabaenaflos—aquae(r=09344)2Studiesondelayedbioluminescentspectrumofanabaenaflos

8、aquaeshowed：1)Becauseanabaenaflos—aquaeislowlevelspecies，thedelayedbioluminescentspectrumofanabaenaflos—aquaewassimple，spectrallinesemittedfromanabaenaflos—aquaewere440nmand575nm2)Chorophyllaplayedanimportantroleinlhedel

9、ayedbioluminescent3Thediscussionthatmetsulfuronmethylinfuencedthepropertiesofultraweakbioluminescenceofanabaenaflos—aquaeelucidated：metsulfuronmethylinfluencedtheintensionofultraweakbioluminescenceandchangedthespectraldi

10、stribution2)metsulfuronmethylmaymoreeffectivelycontrolthegrowthofanabaenaflos—aquaeinlowertemperature4Inordertostudythemechanismofanabaenaflos—aquaeagainstmetsulfuron—methyl，thetrialwascarriedoutbythecomparisonbetweensen