蘋果酸對(duì)內(nèi)蒙古白絨山羊瘤胃發(fā)酵功能及生產(chǎn)性能影響的研究.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學(xué)碩士學(xué)位論文蘋果酸對(duì)內(nèi)蒙古白絨山羊瘤胃發(fā)酵功能及生產(chǎn)性能影響的研究姓名：施玲玲申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：動(dòng)物營(yíng)養(yǎng)與飼料科學(xué)指導(dǎo)教師：孫海洲盧德勛20080601StudyonEffectsofMalateonRumenFermentationandPerformanceofInnerMongoliaWhiteCashmereGoatsAbstractThispaperincludedthreeparts；Thefirsttr

2、ialwasstudiedoneffectofdifferentdosesofmalate(0、3、6、9、12mM／L)onrumenfermentationofcashmeregoatinbatchcultureTheculturesubstratesweretwotypesofdietswithconcentratetoughagermioof3：7and2：8，theaimwastoselectsuitablemalatedos

3、eofsupplement；ParttwowasstudiedontheeffectofmalateonfermentationactivityofforagedietbyRuminococcusflavefaciensinpurecultureInthethirdtrial，12whitecashmeregoatsofhalfsibwithlumenfistulawereusedtodeterminingtheeffectofmala

4、teonrumenfermentationandperformanceofwhichfed3：7and2：8dietTheresultswereasfollows：(1)TheresultsofbatchcultureshowedthattheeffectsofmalateonlumenfermentationweredifferentunderdifferentdietswithforagetoconcentrateratioAcco

5、rdingtoMFAEI，optimalsupplementaldosesofmalatein3：7dietand2：8dietwere6mM／Land9mM／LrespectivelySOtheadditionofmalateindietsofcashmeregoatwere1289／dand1939／drespectivelyinvivo(2)TheresultsofpurecultureofRuminococcusflavefac

6、iensshowedthataddingmalateintheforagedietsimprovedthefermentationactivity，furthermorethegasproduction，pH，theconcentrationofNH3NandTVFA，DMandNDFdegradation，thenumberofcellulolyticbacteriaof2：8dietgroupand3：7dietgroupwereh

7、igherthanthatofgrassgroup(3)WhendifferentratioofconcentratetoroughagewerefedindietofCashmeregoat，concentrationofNH3N，MCPandTvFAiIltherumenwerehigherin3：7dietthanthatin2：8group，whileruminalpHwashigherin2：8dietBothoftwodie

8、ts，addingmalatedecreasedrurilenNH3Nconcentration，lactateconcentrationandA／Pratio，butincreasedlumenpH，concentrationofMCP，acetate，propionate，butyrateandTvFA，butthereWasriosignificantdifference(P005)(4)Numbersofruminalbacte

9、riaandSRuminantiuminCashmeregoatwerehigherin3：7dietgroupthanthatin2：8groupThenumberofcellulolyticbacteriaWashigherwithfeeding2：8dietgroupInthetwodietsofmalatesupplement，thenumberofsuchthreekindsofbacteriaWashigherthantha