baba、meja及bth誘導向日葵抗菌核病的研究馬鈴薯npr1基因的克隆及其表達載體構建

1、 分類號 Q37 U D C 575 學校代碼 10129 學號2010211007 BABA、MeJA 及 BTH 誘導向日葵抗菌核病的研究&馬鈴薯 NPR1 基因的克隆及其表達載體構建 Study of BABA、MeJA and BTH Induced Resistance to Sclerotinia sclerotiorum in Sunflower & Cloning and

2、 Expression Vector Construction of Potato NPR1 Gene 申 請 人：劉雪鋒 學科門類：理 學 學科專業(yè)：遺傳學 研究方向：植物抗病分子生物學 指導教師：白薇 副教授 論文提交日期：二○一三年六月Study on BABA、MeJA and BTH Induced Resistance to Sclerotinia in Sunflower & Cloning and Expre

3、ssion Vector Construction of Potato NPR1 Gene Abstract Sunflower (Helianthus annuus L) is one of the most important oil crops in the world. Sclerotinia sclerotiorum is one of the most serious diseases in sunflower. Until

4、 now, the fully resistant material to Sclerotinia sclerotiorum has not been found in sunflower. Also the safe and effective measure to control Sclerotinia sclerotiorum is not available. Using inducer to induce resistance

5、 in plants to defend pathogens is an effective means. So β- aminobutyric acid (BABA), methyljasmonate(MeJA) and 1, 2, 3-benzothiadiazole - 7- carbothioic acid S- methyl ester (BTH) were used to treat sunflower plants. Th

6、en several important defense- associated enzyme activity were tested. We found that after the above three substances induction, the activities of phenylanine(PAL), polyphenol(PPO) , peroxidase(POD) and catalase(CAT) are

7、higher than those of untreated control. And after inoculation with Sclerotinia sclerotiorum, lesion areas of detached leaves treated with the above inducers were significantly smaller than control. This indicates that BA

8、BA, MeJA, and BTH can induce resistance to Sclerotinia sclerotiorum. Our research laid foundations for further study of induced resistant mechanisms and the application in sunflower production. Potato (Solanum tuberosum

9、L) is the fourth largest food crop in the world, its cultivation area and production is only after wheat, rice, and corn. Potato plants are susceptible to a wide variety of diseases. Systemic acquired resistance (SAR) is

10、 an indicible defense response that produced by local pathogen infection. It confers long- lasting protection against a broad spectrum of microorganisms and is thus a very promising tool to combat these potato diseases.

11、It is well- known that NPR1 (non- expresser of pathogenesis related genes 1) is the master regulator of salicylic acid- mediated SAR, worked as a co- transcriptional regulator. NPR1 can induces the expression of PR genes

12、 and produces resistance to pathogens. The NPR1 cDNA and its promoter segment were cloned from potato sequencing material. The binary vector was constructed and transformed into agrobacterium. This laid foundation for fu