高產(chǎn)玉米肽菌株Bsls532的選育及發(fā)酵條件的研究.pdf

1、UniversityCode：10225RegisterCode：080324DissertationfortheDegreeofMasterStudiesonBsls532CornPeptideMicrobialBreedingandFermentingConditionCandidate：Supervisor：AcademicDegreeAppliedfor：Speciality：DateofOralExamination：Univ

2、ersity：NiuHongyuProfZhangZhiMasterResourcesofForestPlantJune172008NortheastForestryUniversity東北林業(yè)大學(xué)碩士學(xué)位論文AbstractPolypeptidesareconcernedtObiologicalactivematterofeachkindofcellfunctioninorganismPolypeptideshavebecomeabe

3、tternutritionmatterwhichareuncontestablefactatpresent，becausepolypeptidesorproteinhydrolyzedmatterhavemorebiologicalandnutritionalvaluethandissociativeaminoacidorprotein，andtheyareabsorbeddirectlybyhumanbodyintestinesThi

4、spaperdiscussedthatbigmolecularcomproteinwasdecomposedintosmallmolecularpeptidewithmicrobialfermentiontechnologyMicrobialfermentionproducingpeptidewasthatasmicrobegrewandpropagated，proteinwasdecomposed，microbeenzymesyste

5、mwascomplex，wheninscribedenzymecutthebigmolecularproteinintosmallpeptide，modificatoryenzymedecoratedtheexposedhydrophobebondcopolymerthatmadenewproducedsmallmolecularpeptidetastesoftcomparativelySoCOrnpeptidehadlowcost，n

6、obitternessandSOmanymeritswhichwereproducedwithmicrobialmethod，andwhichwasisabettermethodtoproduceCOrnpeptideTherewerefivepartsinstudyingpeptide：(1)thistestchoseonebacteriumfromtheoriginalbacteriumwhichwereTrichodermavir

7、ideLs一01，AspergillusnigerLs一02，BacillusnattoLs03，AspergillusoryzaeLs一04，Bacillussubtilis厶一05，whichhadcapacityofhighproductionproteinenzyme；(2)thechosenbacteriumwasmutagenicbyphysicalandchemicalmethod，andgotanaimbacterium

8、whichhadcapacityofhighproductionproteinenzyme，andoptimizedofitscultivationconditions；(3)thefermentionconditionswereoptimizedbytheresponsesurfacedesignTheresultswereasfollows：(1)BacillussubtilisLs一05waschosedthathadthecap

9、acityofhighproductingalbumenenzymefromtheoriginalbacterium；(2)BacillussubtiIisLs一05wasmutagenicbyphysicalandchemicalmethod，thatwasirradiatedbyUVfor120s，andwasmutagenicbychemicalmethodfor40rainandgotthehighproductingalbum

10、enenzymeBacillussubtilisBsls532；(3)wemadecertainthatthebestculturemediumwere：yeastgrease1％，peptoneO5％，glucoseO5％，NaClO2％，theoriginalpHwasnaturalbyorthogonaltest，thebestculturalconditionswere：therevolutionofsurgingculture

11、casewas180r／rain，culturetemperaturewas34。C，culture，timewas24h；(4)theoptimalfermentionconditionwasconfirmedbyresponsesurfacedesign：afterthematerialWasdealtwimdeamylumanddegreased，crushedto80unit，substrateconcentrationwas5

12、％，primarypHwas80，holdingliquidlOOmlin500mltricomebottle，inoculationquanlitywas10％，culturaltemperaturewas40。C，fermentiontimewas68h，andthepeptideyieldwas8350％Keywords：cornalbumenpower；cornpolypeptides；Bacillussubtilis；ferm