L-組氨酸高產(chǎn)菌株的選育及發(fā)酵條件的優(yōu)化.pdf

1、湖北工業(yè)大學碩士學位論文L組氨酸高產(chǎn)菌株的選育及發(fā)酵條件的優(yōu)化姓名：劉德超申請學位級別：碩士專業(yè)：發(fā)酵工程指導教師：邱雁臨20060501湖北工業(yè)大學碩士學位論文AbstractL—histidinewasan鋤inoacidwithahete∞cycIicimidazole，itwasalsooneofthetwentyaminoacidswhichcomposethehumanproteins，it、vasahalfnecessar

2、yaminoacidwhichequipotentialpointwas759，atthesametime，itextensivelyattacheditselftomanyl(indsofphysiologicaIandbiochemicalprocesswhichincIudingitcomposingr【lanyeLcmentsofS0meb沁chemicalpmcessa11deliminatingoxideaIldattach

3、jngmodulationofimmunj母Nowinourcountrywearedoir堰hardworktopmduseLhistidinebyfe衄entationandwehopethatwecanapplythis妣hniquetoindustryInmisanicleollrcentralworkwaStogetahigherLhistidinepmduceraIldmal(ingthcoDtimizationofbott

4、lefbnmentationconditions：(1)Inthisartcle、vedidsomeresearchofthemeansofsurveyingtheLhistidine，finallywemadesomeimprovcmentswhichwechangedthevolumeofL一|listidine，connrnledasimpleand協(xié)tmeaIlsofPaullycolorimetrybywhichwccould

5、survevtheL—histidineinthefermentatiomlnuid：extracted3mlL—histidinetotlecuvetce，tllenwe舯tmestalldardcurveofLmistidine，arIdtheR‘waSO9998，wecoIlldapplyittolargescalesurveymg(2)WeusedCD，)we6口c把一“m罟缸m卅fc“mS9114aStheoriginalst

6、rain，thenwegettllemutantbypmcesSed塒tllUV、DES、NTG，aIldthencllltivateonthegmdsnatS砌chcon“nedDIhistidincand6MG，finallywegetamutalltstrainnamedN13mli—chcouldproduceL—11istidine56lmg，L，and也eprductwasincreased15270％morethanS91

7、14(3)Inmisartcle、veoptim砌thebottlefenrnentationconditions’finallywedecidedthebestoftheseedsa霉哆was15hours：thecycleof讓站strafm矗Imcntationwas70hours；andwedecidedbestfemlentationcul眥medi眥comprised10％glucose，鋤monium4％suIfate，1

8、5％comsteepliquor，02％ma趴esiumSulfate；aIlewedecidedmebestvolumeoffemlentationaIiquidwas30mlinme300mlbome：alsowedecededthebestextmctedseedSfluidwas75％；thebestoriginalpHofmefemlentationculturemediurn、Ⅳas74；wealsodecidedthebe

9、strotatespeedoftheculturcmachinewas130f／min(4)Bytheoptimizingofthefermentationconditions，、vcmadet11ebestprocessapplyingthestminsS6aIldN15，thenwegetthepmductofLhistidinewas577m∥Land720mg／Lrespectively，andtIletheproduct、va