秸稈降解菌的選育及復(fù)配研究.pdf

1、鄭州大學(xué)碩士學(xué)位論文秸稈降解菌的選育及復(fù)配研究姓名：楊小麗申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：環(huán)境科學(xué)指導(dǎo)教師：王巖20090520鄭州大學(xué)碩士學(xué)位論文AbstractWiththedevelopmentofmodernindustrythehrge—scaleuseofpesticidesandchemicalfertilizers，soilorganictrottercontentweredecreasedAtthesametime，asurp

2、lusofstrawsproblemsismoreandmoreprominentresultinginthebmningofthestrawandSOonpollutingtheenvironmentbutalsoresultedinawasteofresourcesStrawretwninghasbeenprovedtoincreasethesoilorganicrmttercontentandtmiraainthematerial

3、bahnceofhndu∞HoweverstrawreturningdirectlywilladverselyaffectagriculturalproductbncombinedapplicationofhighefficientstrawdecomposingbacteriawithitwaseffectiveInthisstudybasedonstrawcompositionstructureandcharacteristics，

4、throughthestrainselectioncompositionanddomesticatedstrainsinordertobuildhighperformancestrainsofstrawdegradationTheresultswereasfolbws：1Inthisstudyfivestrains，numberedX1一X5，wereisohtedfromrottenstgawcowdungcompostandpadd

5、ysoilAmongthe5strains，XIhadthehigherenzymeactivityandstrawdecompositionefficiencyTheactivitiesofCx，CbC1，F(xiàn)PAandhemicellulaseofX1were536U／mL，697U／mL，95U／mL，116U／mLand23415U／mL，respectivelyThestrawdegradingefficiencyofXlina

6、llstrainswerethehighestreachingupto402％2ThestrainX1Waschosenasthestartstrainformum薩nictreatmentswithultravioletandnitrousacidThemutamY114WasselectedComparedwithX1，TheactivitiesoftheFPA，CxCb，C1，hemicellulaseandstrawdegrad

7、ingofY114wereimprovedby132％l87％，279％，468％，441％and115％，respectively3FourlignindegradingfungiwerechosenaccordingtotheirlignindegradingenzymesactivityL3hadthehighestligninpe∞xnaseactivitythatreachedupto135U／mLSixyeastswasis

8、ohtedfromdistillersyeastandsoiLAmongthenminedJ6couldfermentxybseandglucose，whichWasgoodtoreduceproductinhibitiononcelluloseandhemicellulosedegradation4Usingstrawassolecarbonsource，domesticatedstramsfromforestsoilandbambo

9、oforestsoilWasobtainedbyreslxietivecultureandcombimtionTheslrawdegradingeffmiencyofthedomesticatedstrainswasupto383％AmixturewithY114X2L3J6consistingofcellulosedecomposingfungilignindegradingfungiandyeastwasobtainedbystra