蘇云金芽胞桿菌MB-15菌株特性及其可濕性粉劑的研究.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文蘇云金芽胞桿菌MB15菌株特性及其可濕性粉劑的研究姓名：葛東華申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：微生物與生化藥學(xué)指導(dǎo)教師：王勤英20120526StudyonCharacterizationandWettablePowdersoBacillusthuringiensisMB15StrainCandidate：GeDonghuaAdvisor：ProfessorWangQinyingSpeciality：Microbiala

2、ndBiochemicalPharmacyAbstractBacillusthuringiensisMB1503tMB一151witll1lighinsecticidalactivitiesagainstLepidopteravegetablepestswasisolatedfromsoilinHebeiprovinceInordertomakefurtheI“evaluationonthepotentialofBtMB15asmicr

3、obialinsecticide，thecrystalshapeproteintype，genotype，growthcharacteristicsandtoxicitiesofBtMB15wereobservedandanalyzedInaddition，thewettablepowderofBtMB一15wasdevelopedbyusingitsmixtureofsporeandparasporalcrystalsTheresul

4、tswereasthefollowings1ThebioassayresultsindicatedthatthetoxieitiesofBtMB一15againstHelicoverpaarmigera，Spodopteraexigua，Plutellaxylostella，SpodopteralituraandPierisrapaeallwerehigherthanthatofBtHD1ThesupernatantofBtMB15fe

5、rmentationcouldenhanceremarkablytheinsecticidalactivityofmixtureofsporeandparasporalcrystalsofBtMB15BtMB一15couldproducethebipyramidalcrystalsduringsporulationphaseofgrowthSDSPAGEanalysisrevealedthatBtMB15producedcrystalp

6、roteinsof1300kDand650kDPCRRFLPanalysisshowedthatBtMB一15containedcrylAc，cry2Aa，crylIandvip3AagenesTheseresultssuggestedthatBtMB一15mayhave900dpotentialtodevelopethemicrobialinsecticideforcontrollingLepidopteravegetablepest

7、s2InordertodeveloptheformulationofBtMB一15wettablepowderthecarriers，wettingagents，dispersers，stabilizersandultravioletprotectorswerescreenedTheoptimalformulationWasobtainedbyusingthefreezedriedpowderofthemixtureofsporeand

8、parasporalcrystalsfromBtbIB15asrawmaterial111eingredientsoftheBtMB15wettablepowderformulationwereconfirmedasfollows：50O％BtMB15bacteriumpowder10O％DodecylBenzenesulfonateaswettingagent5O％SodiumSulphonateLigninasdisperser10

9、％XanthanGumasstabilizer1O％skimmedmilkpowderasultravioletprotectant，33O％WhiteCarbonBlackascarrierThequalitydetectionshowedtllatthecontentoftoxinprotein，titersuspensionandwettingtimewererespectively24％，26546IU／mg，74％and123