貓棒束孢菌抗真菌多肽和巨大口蘑抗真菌蛋白的分離純化及特性的研究.pdf

1、中國(guó)農(nóng)業(yè)大學(xué)博士學(xué)位論文貓棒束孢菌抗真菌多肽和巨大口蘑抗真菌蛋白的分離純化及特性的研究姓名：郭永霞申請(qǐng)學(xué)位級(jí)別：博士專(zhuān)業(yè)：微生物學(xué)指導(dǎo)教師：王賀祥20051101Abstract／sar／a力linawasacontaminatingfungusisolatedfromthesubstratewhencdfivmingediblemushroomPholiotanwmekoThefungusinducedabroadinhibition

2、zonewhenintroducedthizoctioniasolani0npotatodextroseagarmediumThroughethanolextraction，absorptiononYPRIImacroporeadsorptionresin，emylacetateextraction，petroleumetherprecipitationandrecrystalli盟fionfromethylacetatefinecol

3、ourlesscrystal，isarfelin，wasobtainedfrommyceliaofZfeltnaTheinhibitoryactivityto尼solaniwasenhancedaftereachsteppurificatedIsarfelinwasbroadspectrum，notonlyexhibitedstrongantifungalactivityagainstseveralphytopathogensbutal

4、sopresentedinhibitiontoAgrobaeteriumrhizogenesK27whichcausedcrowngallofpeach，whereasshowednoinhibitiontomyeeliaofseveralmushrooms1sarfelinexhibitedcytotoxicitytoMBL，L1210andhepG2celllinesandinhibitedHIVIrevelseWanscripta

5、seTheinhibitoryactivityfunctionedstablyonpH49，anditWasheatandlightstable1sarfelinw∞amixtureofisarfelinAandisarfelinBThemolecularweightofisarfelinAWas6694178withthemolecularformulaC361IssOTNs；themolecularweightofisarfelin

6、Bwas6554028withthemolecularformulaCssHss07N5IsarfelinAmadeup412％andisaffelinBmadeup588％1sarfelinAonlyhadaCH2morethanisarfelinBandthestructureofbothWasverysimilarThescanelectronmicroscopyshowedisarfelincausedtheabnormityo

7、fmyceliaofSclerotiniasclerotiorumwhichincludingexcessivebranchingandexpandedhyphaltipWhcreas，isarfelinexhibitednoeffectonthepermeabilityofceilmembraneandrespirationofScle刪nmsclerotionanTherehavebeensomereportsonthebioact

8、ivitycompoundfromTricholomagiganteumandtheantifungalproteinresourcewillbewidenedthroughthepurificationofantifuogalproteinfrom丹icholomagiganteumAnantifungalprotein，trichogin，WasisolatedfromthemusllroomTricholomagiganteumT

9、heprotocolincludedionexchangechromatographyonDEAEcellulose，affinitychromatographyonAffi—gelbluegel，ionexchangechromatographyonCMcelluloseandgelfiltrationbyfastproteinliquidchromatographyonSuperdex75Itwasamonomericprotein

10、withthemolecularIImsof27kDa，TheN—terminalsequenceoftrichogin，QVHWPMF，wasnotfoundinanyreportedantifungalprotein，suchasAlveolarin，Eryngin，andLyophyllumantifungalproteinwhichwerepreviouslyisolatedfrommushroomThebehavioroftr

11、ichoginonthevariousionexchangeandaffinitychromatographicmediabnemployedintheisolationprocedureissimilartothatreportedforotherantifungalproteinsItw∞unabsorbedonDEAEcellulosebutWasabsorbedonAffigelbluegelandCM。celluloseTri

12、choginexhibitedontifungalactivityagainstphytopathogenicfungusFusariumoxysporum，MycosphaerellaaraeMdieolaandPhysalosporapiricolaTheICs0oftheantifungalactivityoftrichogintowardMarachidicolaWasestimatedtobe38028IxM，andfurth