桃離體培養(yǎng)及葉片再生體系的建立.pdf

1、V0：’7177密級：論文編號：中國農(nóng)業(yè)科學院學位論文桃離體培養(yǎng)及葉片再生體系的建立PlantRegenerationofPeachfromLeafletCultured玉lVuro碩士研究生：閏承璞指導教師：王志強研究員申請學位類別：農(nóng)學碩士專業(yè)：果樹學研究方向：生物技術育種培養(yǎng)單位：中國農(nóng)業(yè)科學院研究生院中國農(nóng)業(yè)科學院鄭州果樹研究所提交日期2006年12月AbstractInordertooptimizetissuecultures

2、ystemofpeach(PrunuspersicaL)andtoimproveplantletregenerationrateandtoestablishhighefficientplantregenerationsystemfromleaves，leaveswasusedasexplantsonthebasisofestablishmentofarapidtechniquesystemofstableandhighefficient

3、micropropagation，Wildpeachseedling[Prunuspersica(L)Batsch]Gansutao僻kansuensbRehd)，Sunagowase陋persica(L)Batschj，Shuguang∽persicavarnectarinaMaxim)andZaolupantao(尸persicavarplatycarpaBailey)wereusedastestedmaterialsTheresu

4、ltswereasfollows：(1)Arapidtechniquesystemofmicropropagation／nVitroculturewasestablishedTheeffectofsamplingtime，basicmedia，culturalconditionandthemethodofdisinfectiononprecultureweredistinguishedTheresultswereasfollows：As

5、toShuguang，Sunagowase，Wildpeachsideling，andZaolupantao，explantsweresampledoilMarcia，April，MaySeptemberandOctobertherateofgerminationwasveryhigh，buttoGansutao，sampledfromMarchtoOctobertherateofgerminationkepthigherthan79％

6、andnosignificantdifferenceIdealpre—culturemediawasLPmediatherateofgerminationwas210％ofMSmedia；lightintensityof1000Ixwasbeneficialtoexplantsgermination；theprojectofdisinfectioninwhichtherateofcontaminationwas2％andinjuryve

7、rylittlewasprovedupTheresearchwasfocusedonbasicproliferationmediaand3typesofNAAandBAPcombinationorTDZandNAAcombinationandpHincontinuous3timessubcultureInconclusion，theoptimummediawasLPmediawith075mg／LBAPandO3m【g／LIBAforS

8、huguangSunagowaseWildpeachsidelingandZaolupantao，butSHwithO75mg／LBAPandO_3mg尼／BAforGansutaoThefavorablepHforproliferationculturewas5458forpeach(2)AhighefficientregenerationsystemfromleaveswasestablishedThetechniqueandcon

9、ditionofadventitiousshootfromleaves加VitroculturewerestudiedTheeffectsof3kindsofdifferentsourceofleaves，regenerationmedia，leavesmaturityandmaterialonthepositionofcrownofatreeonregenerationwereanalyzedTheresultsshowedthat，

10、withidealexplantsfromthetenderleavesofadventitiousshoottheregenerationratewasthehighesM5％Butfromproliferatedleavesorpre—conditionedleaves，theregenerationratewasunder33％ThebetterregenerationmediawasSHTDZ30mg／LKT03m∥LThere

11、generationrateof10—15cmlongfullyexpandedleaveswas3timesthan05一l0cmlongThematerialfromthebottomofcrownofatreewaspropitioustoadventitiousshootregenermionfromleavesAlltheplantletsdevelopedintocompleteplantswithnormalmorphol

12、ogicalcharacteristics(31TheadventitiousshootswererootedandplantletswereobtainedTheeffectsofculturalcondition，basicmediaandhormoneontherootingwerestudiedTheresultswereasfollows：ARerl0daysof3001xlightintensitytreatmenttran

13、sferredto25001xlightintensityandculturedfor20d，amountofeffectiverootswere386pershootandtherateofrootingwas100％ThebestrootingmediumwasI／2LPsupplementedwith1Omg／LNAAand10mg／LIBAKeyWord：peach，PrunuspersicaL，adventitiousshoo