利用苦瓜幾丁質酶和苜蓿防御素基因提高水稻抗病性的研究.pdf

1、EnhancedResistancetoBlast(抓心刀aPthegrisea)SheathBlight(Rhizoctoniasolani)inTransgenicIndicaRicebyConstitutiveExPressionofChitinaseandDefensinGeneADissertationSubmittedtoSouthwestUniversityInPartialfulfillmentoftherequirem

2、entforthedegreeofDoctorofPhilosoPhyinAgronomybyLiPingDiseiPline:CroPGenetiesandBreedingDirection:RieeMoleeularBreedingDissertationSuPervisor:Prof.GuanghuaHeChongqingP.R.ChinaDecembef，2008西南大學博士學位論文旦旦旦旦旦里旦旦旦旦旦旦旦里旦旦旦旦旦旦口里目

3、旦3.3.1植物表達載體的構建.......................................................................……373.3.2緒恢35的農桿菌介導遺傳轉化體系的建立.................................……423.4討論................................................................

4、.............................................……503.4.1對愈傷組織誘導和繼代的影響.......................................................……503.4.2對愈傷組織分化再生的影響...........................................................……513.5小結.........

5、....................................................................................................……52第四章植物防御素a柳FP基因的酵母體外表達及體外抑菌研究...................……544.1實驗材料..........................................................

6、...................................……，.……544.1.1菌株和主要載體...............................................................................……544.1.2主要藥品與試劑......................................................................

7、.........……544.1.3主要儀器設備...................................................................................……554.1.4主要培養(yǎng)基和緩沖液配方...............................................................……554.2實驗方法...................

8、..................................................................................……564.2.la柳FP基因的酵母表達載體的構建...............................................……564.2.2重組表達質粒的提取................................................

9、.......................……564.2.3質粒的線性化及去磷酸化處理.......................................................……564.2.4畢赤酵母感受態(tài)細胞的制備及電轉化...........................................……574.2.5酵母轉化子的G418篩選...............................

10、.................................……574.2.6重組蛋白的誘導表達.......................................................................……584.3重組蛋白的抗菌活性檢測.........................................................................……584.3

11、.1水稻紋枯病的抗性檢測................................……，............................……584.3.2稻瘟病的抗性檢測...........................................................................……584.3.3白葉枯病的抗性檢測...................................

12、....................................……584.4結果和分析..…，..........................................................................................……594.4.la抑FP基因的擴增和載體的構建...................................................……5

13、94.4.2重組質粒對畢赤酵母GSI巧的轉化..............................................……594.4.3PPICgK一a洲FP誘導表達的鑒定.....................................................……604.5討論...............................................................

14、..............................................……634.6小結...............……，.，.....................................................................................……64第五章轉基因水稻的抗病檢測...........................................

15、..................................……655.1實驗材料與方法.........................................................................................……655.1.1實驗材料......................................................................

16、.....................……655.1.2實驗方法...........................................................................................……665.2結果與分析...................................................................................