抗甜菜壞死黃脈病毒(BNYVV)單鏈抗體在煙草中的表達以及BNYVV易感材料——番杏的組培的研究.pdf

1、東北大學(xué)碩士學(xué)位論文抗甜菜壞死黃脈病毒(BNYVV)單鏈抗體在煙草中的表達以及BNYVV易感材料——番杏的組培的研究姓名：王文星申請學(xué)位級別：碩士專業(yè)：應(yīng)用化學(xué)指導(dǎo)教師：劉聲遠曾群祉2001.2.1Absti’actNbenthanliauaand’Fetragoniaexpansawerechosenasnlaterialtostud)’tilediseaseresistancecharactersofsinglechainantib

2、ody(scFv)againstbeetuecloticyellowveinvirusrBNYVV)Thevectorforexpressinganti—BNYVVscl、，inplantswasCOllStluctedand“ansformedjlltONbenthamimlabyleafdiscnlethodTransfiqlned1)lantswereselectedonmediacontainingkanamycin。rilee

3、xpressionvectors；1CIeoftwokinds：OllewasplaslnidpBllSPcontainiugsignalpeptidesequence：tileotllewaspBSPnotcoutainingsignalpeptidesequenceIdentifiedbydot—blottingandsouthern—blottiug3transgenicplantstransforuledwithpBHSPaud

4、ltlausgenicplanttrailsformedwithpBSPwereobtaiuedThroughSDSpolyacrylamidcgelelectrophoresisoftotalplantproteins，aspecialbandappearedontiregelsof3trausgenicplantstransfornledwithpBSPanditslnolecularweightwasabout2U000Dalto

5、n，whichwasconsistentwithtileexpectednlolecularweightof29883ButtIlegelof1tlansgenicplanttrailsfornledwithpBSPdiduothavetirespcdalbaudthesesllowedthatsignalpeptideaffectedexpressinglevelofthesclVgeueWealsoexIqoredtilebiolo

6、gicalactivitiesofscFvandtilediseaseresistantcharactersoftlansgenicI)hlats，althoughtheywereveryprilnalyAlsocarriedoutinthisstudywcresubcultureandstemregeuerationofTctlagoniaexpansa，111tilehopethattheyCallbeusedonbeeti11ti

7、lefulure’lheresultsofsubcultureexperimentindicatedthatvitritbrillseedlingscouldreveltto110IlnaIseedlingsbyreducingtemperatureandlesseningllumidiUtileculturevasesSteinregeneratiouexperinlentsexploredtheeffectofplauthorlno

8、neanddarkincubatiouOilcalliandantibioticsensitivitydeterminationwasalsocarriedoutTlneekindsol’calliwereinducedthrouglldifferentlevelsofllorlnonecolrcentration0nekiudwaswhiteandtightlycolnpacled，anditCallbesubculturedfora

9、longtinlewithoutdj他renlialiouAuotheronewaswlliteandsliglltlycolnpacted，withconvexpointsanditcal!onlydi仃erentiateilltOwhitedowuyrootYetanotherwaswhite，Ioose，withlnany101／3，cuds，lookedlikecaulifloweranditCalldifferentiatei

10、utogreeuseedliugafterlongtimecultivationComparedtolightincubatioudarkincubationhadnoeffectontiletimeofiuducingcallibuttilecallitreatedwithdarkincubationgrewnmchbetterAlitibioticseusitivitydeterminationresultssuggestedtha