葡萄高效再生體系的建立研究.pdf

1、西北農(nóng)林科技大學碩士學位論文葡萄高效再生體系的建立研究姓名：張宗勤申請學位級別：碩士專業(yè)：果樹學指導教師：王躍進20040501AbstractExperimentsofinitiationandregenerationofvarietiesofgrapevinesinvitrowereperformedTenand12ofall19varietiesinvolvedinthisstudywereobtainedadventitious

2、budsand／orembroidsrespectivelythroughdistinguishedliquidoragarsolidmediacombinedwithsomekindsofconcentrationsandcombinationsofplantgrowthregulatorsEffectiveregenerationexperimentaltechniqueseriesofdifferenttypesofgrapevi

3、nevarietieswereestablishedviastudyingoffactorsaffectingorganogenensisandembryogenensis，formeetingfurthergenetictransformationoperations1Initiationofgrapevines：Sampleseasonwasthecriticalfactorinfluencingtheinitiationeffic

4、iencyThelowestcontaminationwasobtainedatspring，ApriltoMay，andthehi曲estwasataugustThesuitableexplantswerethetop1—3nodesectionsofplants(apexexcised)comparedwiththe4N6nodalpartsDedicateterminalswerenotexplantsneededbecauseo

5、ftheirhigherbrowningprobabilityTwicesterilizingtechnique、vith48hintervalwanthetopefficiencyofinitiationof90％，whichwas30—40percenthi曲erthanregularonestepsterilizationsequence2Embryogenesis：Theappropriatemediumfordediffere

6、ntiationWasNN692，4一D20mg／LBA02mgrLforlea￡shootandrootsegmentexplants，WasNN692，4一D20mgmBA20mg／LforpetiolecutEmbryogeniccalluswereinitiatedonNN692，4一D20mg／LBA02mg／LofleafexplantsEmbroidswereobtainedon1／2BsBA50me／1IBAO1mg，L

7、，ofThompsonSeedless，F(xiàn)lameSeedless，Delight，OlmoSeedless，andZuoshan一1EmbryogeniccalluscouldbemaintainedonNN6924D01medLBA02mg／LTheappropriatemediumforembroidmaturationWas1t2BsBA02mg／LCH100mg／L3Organogenesis：Adventitiousbuds

8、wereobtainedof10of19varietiesinvolved，ThompsonSeedless，F(xiàn)lameSeedless，Dellght，Youngle，RedGlobeeta1Theoptimalmediumforleafdiscexplantsorganogenensisfor‘ThompsonSeedless’and‘RubySeedless’were1／2MSBA50mg／LTDZ01mg，LIBAO05mg／L

9、，for‘RedGrape’WasNN69BA50mg／LTDZ02mg／L，for‘FlameSeedless’was1／2MSBA10meteTDZ002medLThehighestregenerationratioWasobtainedat3667％of‘Rubyseedless’leafexplants4Rooting：Rootedplantscouldbeobtainedofplantletsregenerated，of15—