毛細管電泳滲流流體力學進樣方式的研究.pdf

1、重慶大學碩士學位論文毛細管電泳滲流流體力學進樣方式的研究姓名：孫冠蕓申請學位級別：碩士專業(yè)：物理化學指導教師：夏之寧2000.11.1墾壅叁：：i蘭塑堂壁壘奎壘壁!!墜壘竺!ABSTRACTCapillaryElectrophoresis(CBlisnotedforitshighresolutionandsmallvolumeofsampleIoattainthehighefficiencycapabilityofCE，itisvery

2、importanttointroduceasmallvohuneofsampleintothecapillarypreciselyandreproduciblyCurrently，themostcommonlyusedsampleinjectionmethodsarehydrodynamicandelectrokineticinjection’lherearesomeotherinjectionmethodshavebeendevelo

3、pedsuchason，columnfractureforsampleintroductionelectricsamplesplitter，diffuseinjectionandoncolumnopticalgatinginjectionThemostremarkablelbaturesabouthydrodynamicinjectionareperfectreproducibilityandno—discriminationbutth

4、eequipmentofthismethodiscomplexandexpensiveAlthoughelectrokineticilljectionisthemostsimpleinjectionmethodjnCEduetothediscriminationofcomponent，itwouldbehamperedTileothermethodshavetheshortagemoreorlessInthispaperonenovel

5、injectiontechnologyhasbeendevelopedontheprincipleofelectroosmoticflow(EOF)，whichiscalledeleclroosmolic’hydrtMynamicinjection，andthen，theelectroosmoticflowvelocityhasbeenmeasuredsuccessfullybyusingthesetofinjectiondevice，

6、whichiscalleddirectO’eleclroosmotic1lo’velociO’mPaswemP“fmethodAtlastwiththewayappliedtomeasuringEOFavarietyoffactorsthatcaneffecttheelectroosmosishavebeenstodledAccordingtodesigningandprocessingthekeypart—connector。thed

7、evicehasbeenfinishedinlab，andthesamplehasbeenintroducedinthecapillarysuccessfullyThroughSelectingandoptimizingtheparameterofinjection，suchaselectricfieldintensity，injectioncapillaryinnerdiameterandbuffertheinjectionrepro

8、ducibilityhasreachedat00559％fn56)RSD，theleastsamplevolume274n1AlltheseresultswerecoincideorexceedwiththedatainpreviousliteraturesComparedwiththeneuralmarkermethod，thedirectlyelectroosmoticflowvelocitymeasurementmethodhas

9、acertainextentfeasibilityandveracityAlongwiththefoundationoftilenewmeasuringmethod，theinfluencingfactorsonEOFvelocity，suchaselectricfieldintensitycapillaryinnerdiameterbufferspH，concentrationandionstrengthmodifiedadditiv

10、e，havebeenstudiedFinally，comparedwiththeotherinjectionmethods，eleclroosmotichydrodynamicinjectionhaslittlediscriminationelectrokineticThroughexchangingthedirectionol’EOFininjectioncapillaryanodiscriminationhydrodynamicin

11、jectionmethodhasbeenrealizedAnewsampleinjectionmethodincapillaryzoneelectrophoresisisdevelopedinthispaperThemethodisfeasibleandhasno—discriminationwithitssimpledeviceEspeciallytheendofinjectioncapillarycanbeputintoanyare