氨基酸酶促反應(yīng)中光替代酶的研究.pdf

1、廈門大學碩士學位論文氨基酸酶促反應(yīng)中光替代酶的研究姓名：王細生申請學位級別：碩士專業(yè)：分析化學指導(dǎo)教師：蔡維平1999.7.1／AbstractThisdissertationconsistsofthreechapters，whicharctheadvanceinphotochemicalfluorimetry0CF)，studyonthephotonasaminoacidoxidasesubstitutestudyandapplica

2、tiononthephotonsubstitutionforTyrosineperoxidaseInchapter1thedefinitionandtheoryofPCFwerediscussedandrecentaI“ancesandapplicationsofPCFwerereviewedAtthesametimeweintroducedsomeimportantmimeticenzymesystem，mimeticenzymead

3、vancesandapplicationsinrecentyearsOntheintroductionofsomeworkofthephotonasenzymesubstitute，weputupourconceivationofthethesisInchater2thepossibityofphotonsubstitutionforaminoacidoxidaseWasstudiedAtfirst，westudiedthephoton

4、asPhenylalaninesubstituteTheresultsshowedthatthephotonsuccessfullysubstitutedforPheoxidase，whichcausePhctodecomposeandproduceN也andH202Inthepresenceofheminwhichisashorseradishperoxidase(HRP)substitute，thesubstrate—HPA，whi

5、chisnon—fluorescent，wasoxidizedbyH202toproducefluorescentdimers，thusanewdeterminationofPheWasfoundedOnthisfoundationwesuccessfullyusedphotonsubstitutionforGlutamicacido)【idaseUsingheminasHRPsubstituteandsubstrateHPAtodet

6、ectH202andNesslertodetectNH3，afluorimetrymethodandaspectrometrymethodwerefoundedtothedeterminationoftheGluInchapter3，thestudywasconcentratedonthephotonasTyrosineperoxidasesubstituteWefoundthatunderirradiationTyrCanproduc

7、eserfredoxreactionandproducefluorescentdinlersThephotochemicalreactionproducthadthesamefluorescentspectraasthoseoftheproductsinenzymereactionThemethodWasdevelopedforthedeterminationofTyrinthebeerandthevaminwhichiscompose