雞腸道乳酸菌對抗IBV感染CEF細(xì)胞抗氧化的研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文雞腸道乳酸菌對抗IBV感染CEF細(xì)胞抗氧化的研究姓名：盧巍申請學(xué)位級別：碩士專業(yè)：動(dòng)物醫(yī)學(xué)；預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：魏萍20120606ABSTRACTTheAntioxidationV缸ianceofCEFwithChickenIntestinalLactobacillusafterIBVInfectionAbstractBacteriumofLactobacillusiSbenefittothehealthofh

2、umanandanimalsItcouldkeeDmebalanceofenterobacteriaregulateimmunization，promotemetabolism，andSOOilCurrentlytherealelorsofresearchreported廿1atLactobacillusitsmetabolicproductsanditscellfreeextracthadthefunctionofantilipidp

3、eroxidativeandclearperoxidehydroxyradicalRecentstudyconformedmanydiseaseshadrelationshipwithfreeradicalfreeradicalhadbeenaissueofthreatingtheheathofhumanandanimalsConsequentlyantioxidantactivityinvitroandeffecttoantioxid

4、aseinCEFinfectedwithIBVofLactobacillusWaSstudiedtoprovetheantiinfectionviralactionContentsandmethods：1nleDPPHfreeradicalclearance，hydroxyradicalclearanceandreductionabilityof20strainofLactobacilluswerecompared，whichwerei

5、solatedfromchickenintestines26strainsofLactobacilluschosenfor廿leirhigherantioxidantactivitywereusedforfollowingtrialTolerancetogastricjuiceandbilesaltsofinmanualsimulationgastrointestinaltractconditiondetectingthecontent

6、sofSOD，GSHPxandMDAinCEFinfectedbyIBVResult：1AIltheisolatedhadhi曲antioxidantactivityandreductionabilityDPPHclearanceWaS57％“62％HydroxylRadicalclearanceWaS43％～94％L3，L7，L9，L10，L12andL17hadhigherantioxidantactivitythantheothe

7、r2The6strainsofhaddeterminatetolerancetobilesaltsandgastricjuiceininmanualsimulationgastrointestinaltractconditionTheresultindicatedthatLactobacilluscouldplaynormalfunctioningastrointestinal，whichmighthavepotentialapplic

8、ationvalueIBVcausedoxidativedamagebyreducingSODandGSH—PxandincreasingⅣDAinCEFLactobacillusmetabolitescouldincreasetheactivityofSODandGSHPxtopromoteantioxidantactivityofCEFwithoutinfectedbyIBVAndcontentsofMDAinnormalCEFWa

9、slowerthanCEFinfectedbyIBVWhenLaetobacillusmetabolitesandIBVaddinCEFatthesametime，theactivityofSODWasevidentlyraisedthantheCEFaddedIBVonly(POoo，contentsofMDAevidentlyreducedthantheCEFaddedIBVonly(P001)，buthigherthancontr

10、olgroupNodifferencesintheGSHPxactivitybetweenthetwogroupsItindicatedthatSODplaythemainantioxidantactivityatthisconditionTheresultmanifestedthatthe6strainsofLactobacillusraisedthetotalanti0)【idantactivit，evidently(P001)，a