快速檢測食品中磺胺二甲基嘧啶殘留的膠體金免疫層析方法的建立.pdf

1、南昌大學(xué)碩士學(xué)位論文快速檢測食品中磺胺二甲基嘧啶殘留的膠體金免疫層析方法的建立姓名：林曉麗申請學(xué)位級別：碩士專業(yè)：食品科學(xué)指導(dǎo)教師：賴衛(wèi)華；熊勇華20110616AbstractABSTRACTSulfadimidineiSoftenusedforthepreventionandcontroIofanumberofveterinarydiseasesAsaresult，sulfadimidinecanbepresentinfoodpro

2、ductsofanimaloriginSulfadimidineresiduesinfoodandanimaltissuesmaybepresentinminuteconcentrationsbutmayposeahealththreattoconsumersTherefore，itisimportanttOestablisharapidandsimplemethodforthedetectionofsulfadimidineresid

3、uesinmeatandotheranimalby—products(honeyandeggs)usedforhumanconsumptionThecolloidalgoldstripofsulfadimidinedescribedinthisstudywasdevelopedbymeansofthecompetitiveimmunoassaymechanismThecompetitorofsulfadimidine(sulfadimi

4、dine—BSAconjugate)wasimmobilizedtothedefineddetectionzoneonanitrocellulosemembranewhichactedasthecapturereagent，andthemonoclonalantibodyagainstsulfadimidinewasconjugatedtocolloidalgoldparticleswhichservedasthedetectionre

5、agentforthepreparationoftheonestepstripstodetectsulfadimidineWiththismethod，thedetectionlimitofthestripWas10ng／mLfordetectingsulfadimidineinlessthan5min，whichhadlowcrossreactivitiestoothersulfonamidesThestabilitytestindi

6、catedthattheteststripscanbestoredatroomtemperaturefor18monthsThestripscouldbeusedforrapiddetectionofsulfadimidineresiduesinmanykindsoffoodsamples，suchaspigmuscle，pigliverpigkidneychickenmuscle，egg，cattlemuscle，fishmuscle

7、，shrimpmuscleandhoneyThesefoodsampleswerespikedwithsulfadimidineTheacetonitrilewasusedastheextractsolvent，andthematrixeffectcouldbeeliminatedbydilutionwithPBTheLODofsulfadimidineinninesampleswerelowerthanmaximumresidueli

8、mitofChina(MRL100lag&g)Thismethodissimple，sensitive，rapid，unexpensive，andspecific，whichcouldbewidelyusedforon—sitedetectionofsulfadimidineresiduesinanimalfoodsincludingpigmuscle，pigliverpigkidneychickenmuscle，egg，cattlem