甲萘威、乙霉威和克百威與DNA的相互作用.pdf

1、中國農(nóng)業(yè)大學碩士學位論文甲萘威、乙霉威和克百威與DNA的相互作用姓名：張立金申請學位級別：碩士專業(yè)：農(nóng)藥學指導教師：閔順耕孫英20040601AbstractItiswellknownthatpesticidesoftenhavebiologicaleffectsAsoneofpossibletargets，DNAcanbeattackedbythemoleculesofpesticides，whichmaybringgeneticin

2、formationsomechangesSoitisimportanttostudythemechanismoftheirinterteractionsTheinteractionmodesbetweencalfthymusDNAandcarbaryl，diethofencarb，carbofuranarestudiedbyfluorescenceandUVspectraTheresultsshowthat：1Thereactionco

3、nditions，includingconcentrationofpesticides，PHofsolution，temperatureofsolution，reactivetimes，polarityofsolventandionicintensityofsolutionallhaveeffectsontheinteractionsbetweenpesticidesandcalfthymusDNA2Thequenchingconsta

4、ntsatdifferenttemperaturesarewellconcludedthatcalfthymusDNAleadtothestaticandfluorescentquenchingofpesticides：boththeshiftsoftheirfluorescenceandU1，spectraandthequenchingofK4[Fe(CN)6]indicatethatthemoleculesofthepesticid

5、esintercalateintothetwinscrew’sstructureofcalfthymusDNA：thethermodynamiccalculatingdemonstratesthatthereexistsVanderW姐1sForcebetweenthemoleculesofpesticidesandcalfthymusDNAAdditional，thepolarityofsolventaffecttheinteract

6、ionsbychangingthestructureofcalfthymusDNA，butionicintensitydoesnotchangetheinteractions3、ThebioactivitiesofpesticidesaredirectlyproportionaltoquenchingconstantsBioactivitiesofpesticidesCOllieoutastheresultsofhydrophilicp

7、roperties，areasofplanarstructures，varietiesofsubstitutedproups，electrophilicpropertyofsubstitutedproups，positionofsubstitutedproups，andspatialstructures4、Consideringthesystemamongpesticides，ctDNAandethidiumbromide，thelat

8、tertwocompoundscanbothlendtothefluorescentqucheningofpesticidesBymeansofchangingthespatialstructureofcalfthymusDNA，thepolarityofsolventandionicintensityofsolutiondecreasethenumberofpositionsthatpesticidesandethidiumbromi