魔芋中神經(jīng)酰胺類物質(zhì)的提取工藝研究.pdf

1、分類號UDC密級學(xué)校代碼10497劣禪掛聲大穿學(xué)位論文魔芋中神經(jīng)酞胺類物質(zhì)的提取工藝研究Studyonextractionofeeramidehomologfromkoniac目文目題英題研究生姓名張威娜指導(dǎo)教師姓名一滬斯重遺立職稱崖迷立一學(xué)位一博土一一一單位名稱理學(xué)院化學(xué)系郵編430070申請學(xué)位級別理學(xué)碩士學(xué)科專業(yè)名稱無機(jī)化學(xué)論文提交日期2011年4月論文答辯日期2011年5月學(xué)位授予單位武漢理工大學(xué)學(xué)位授予日期答辯委員會主席香曦評

2、閱人j遴嫉2011年5月武漢理_人學(xué)碩十學(xué)位論文AbStractKonjae15akindofrawPlantmaterial，whichisusedasadrugingredientaswellasafoodtoeat.Ceramideshavefunetionssuehasmoisturizing，whiting，anti一aging，anti一tumorandantivirus.TheyarewidelyaPPliedinfood

3、，medicineandmakeuPindustries.Withthekonjaeastherawmaterial，thisartielemainlytalksabouttheextraetionconditions，seParationandPurifieationmethodsandfunetionalexPerimentsofeeramidesfromkonjac.Atthesametime，aPreParatyanalysis

4、identificationofthekonjaeeeramideswasdonebyIR，UV，TLCHPLC.Theresultsareasfollows:1.UseSoxhetextraetiontesearehtheextraetioneonditions.Theefl七etsofsinglefactorstoextraetionrateofeeramides，suchasextraetiontemPerature，ratioo

5、fliquidtomaterial，theeoneentrationofsolventethanolandtimewereinvestigated:ThebestteelmiealeonditionsfeeramidesextraetionwereobtainedthroughorthogonalexPerimentsasfollows:temPerature92OC，95%ethanol，time12handliquidmateria

6、lratiol:5.Inthiseondition，extractionrateofeeramideswas1.46%.2.ResearehingseParationandPurifieationmethodsofceramidesbySilieaColumnChromatograPhy.TheresultsoftheexPerimentsshowedthat:seParationandPurifieationofeeramideswe

7、reeffeetedbythefiowrateandthePolarityoftheeluantandtheamountofthesamPle.Theflowrateoftheeluantwas0.8mLmin，theamountoftheSamPlewas0.79，theeluantwasV(ethylaeetate):V(Petroleuln)=7:3.Undertheseeolzditions，tlleseParationcffc

8、etwasgood.3.PreParatyanalysisandidentificationofthekonjaeeeramideswasdonebyIR，UV，TLCandHPLC.TheinfraredmaPPingsPectrometercamedtheeharacteristiePeakofthefunetiongrouPsoftheeeramides，whiehdemonstratesthatthePurifiedsamPle

9、eontainsceramidestheTLCeertifiesthattheProduetsareeeramides:thestardsamPleProductofeeralnidesareanalyzedbyHPLC.ThroughtheeolnParetheHPLCgraphsofstalldal‘dsamPleandPz勸duet，wefoundthattlieceramideswereeozitainedinthepurifi