魔芋中神經(jīng)酰胺類物質的提取工藝研究.pdf

1、分類號UDC密級學校代碼10497劣禪掛聲大穿學位論文魔芋中神經(jīng)酞胺類物質的提取工藝研究Studyonextractionofeeramidehomologfromkoniac目文目題英題研究生姓名張威娜指導教師姓名一滬斯重遺立職稱崖迷立一學位一博土一一一單位名稱理學院化學系郵編430070申請學位級別理學碩士學科專業(yè)名稱無機化學論文提交日期2011年4月論文答辯日期2011年5月學位授予單位武漢理工大學學位授予日期答辯委員會主席香曦評

2、閱人j遴嫉2011年5月武漢理_人學碩十學位論文AbStractKonjae15akindofrawPlantmaterial，whichisusedasadrugingredientaswellasafoodtoeat.Ceramideshavefunetionssuehasmoisturizing，whiting，anti一aging，anti一tumorandantivirus.TheyarewidelyaPPliedinfood

3、，medicineandmakeuPindustries.Withthekonjaeastherawmaterial，thisartielemainlytalksabouttheextraetionconditions，seParationandPurifieationmethodsandfunetionalexPerimentsofeeramidesfromkonjac.Atthesametime，aPreParatyanalysis

4、identificationofthekonjaeeeramideswasdonebyIR，UV，TLCHPLC.Theresultsareasfollows:1.UseSoxhetextraetiontesearehtheextraetioneonditions.Theefl七etsofsinglefactorstoextraetionrateofeeramides，suchasextraetiontemPerature，ratioo

5、fliquidtomaterial，theeoneentrationofsolventethanolandtimewereinvestigated:ThebestteelmiealeonditionsfeeramidesextraetionwereobtainedthroughorthogonalexPerimentsasfollows:temPerature92OC，95%ethanol，time12handliquidmateria

6、lratiol:5.Inthiseondition，extractionrateofeeramideswas1.46%.2.ResearehingseParationandPurifieationmethodsofceramidesbySilieaColumnChromatograPhy.TheresultsoftheexPerimentsshowedthat:seParationandPurifieationofeeramideswe

7、reeffeetedbythefiowrateandthePolarityoftheeluantandtheamountofthesamPle.Theflowrateoftheeluantwas0.8mLmin，theamountoftheSamPlewas0.79，theeluantwasV(ethylaeetate):V(Petroleuln)=7:3.Undertheseeolzditions，tlleseParationcffc

8、etwasgood.3.PreParatyanalysisandidentificationofthekonjaeeeramideswasdonebyIR，UV，TLCandHPLC.TheinfraredmaPPingsPectrometercamedtheeharacteristiePeakofthefunetiongrouPsoftheeeramides，whiehdemonstratesthatthePurifiedsamPle

9、eontainsceramidestheTLCeertifiesthattheProduetsareeeramides:thestardsamPleProductofeeralnidesareanalyzedbyHPLC.ThroughtheeolnParetheHPLCgraphsofstalldal‘dsamPleandPz勸duet，wefoundthattlieceramideswereeozitainedinthepurifi