多通道串聯式壓電傳感器用于抗生素后效應和防突變濃度的研究.pdf

1、湖南大學碩士學位論文多通道串聯式壓電傳感器用于抗生素后效應和防突變濃度的研究姓名：王洪燕申請學位級別：碩士專業(yè)：分析化學指導教師：何鳳姣20080625多通道串聯式壓電傳感器用于抗生素后效應和防突變濃度的研究AbstractAntibioticresistanceisbecominganincreasinglyseriousproblemwiththeindiscriminateandinappropriateuseofantibiot

2、icsToslowthedevelopmentofresistance，scientistsinclinicalsettingsfocusontheestablishmentofchangethedoseormethodforthecharacteristicsofthenewtreatmentstrategyandputforwardsomenewtheory，whichisquitenoticeableiSthatpostantib

3、ioticeffect(PAE)andantibioticsmutantpreventionconcentration(MPC)PAEandtheMPCcanobjectivelyreflecttheefficacyofantibiotics，andtheyhavebeenproposedasanewguideforphysiciansintheselectionofappropriateadministrationregimensMu

4、ltichannelseriespiezoelectricquartzcrystal(MSPQC)sensorhasbeenextensivelyusedinrapiddetectionofmicroorganismsfield，andthismethodissensitiveandrapidMSPQCmethodwasappliedtostudyforPAEandMPCPAEandMPCdetectedbyMSPQCisthenewe

5、videnceforcliniciantodirectpatienttotakemedicineThenewmethodsbroadentheirapplicationindetectionofmicroorganismsfieldThemaincontentofthisthesisisasfollow：(1)Anovelmethodwasproposedfordetectionofpost—antibioticeffect(PAE)b

6、yMSPQCThePAEofampicillin，cefotaximeandgentamicinatlx，2xand4xMIConEscherichiacoliATCC25922，PseudomonasaeruginosaATCC27853andStaphylococcusaureusATCC29213werestudiedbyproposedmethodForcomparing，theclassicmethodofviablecoun

7、tsonagarwererunatthesametimeTheexperimentalresultsshowedthatMSPQCmethodhadagoodagreementwiththecomparingmethodGentamicinproducesthelongestPAEsforgramnegativeorganisms；Beta—lactamantibioticsproducelittleornegativePAEwithg

8、ram—negativeorganismsProposedmethodissimple，rapid，andtheinformationcanberecordedinrealtimePAEdetectedbyMSPQCisanewevidenceforcliniciantodirectdosage，dosingtimeintervalanddosingfrequency(2)MSPQCmethodwasproposedfordetecti

9、onofcombinedPAEThecombinedPAEcanbecalculatedbymeasuringthedifferenceoftheFDTbetweenthecontrolandtreatedgroupPAEofgentamicinincombinationwithcefotaxime，ciprofloxacinonEscherichiacoliATCC25922，PseudomonasaeruginosaATCC2785

10、3werestudiedbyMSPQCForcomparison，theclassicmethodofviablecountsonagarwererunatthesametimeTheexperimentalresultsshowedthatMSPQCmethodhadagoodagreementwiththecomparingmethodThecombinationofgentamicinandcefotaximehadanaddit