丙谷胺、塞來昔布對(duì)人胃癌細(xì)胞株BGC-823的抑制作用.pdf

1、遵義醫(yī)學(xué)院碩士學(xué)位論文丙谷胺、塞來昔布對(duì)人胃癌細(xì)胞株BGC823的抑制作用姓名：方興國申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：消化內(nèi)科指導(dǎo)教師：趙逵20090501遵義醫(yī)學(xué)院碩七學(xué)位論文內(nèi)谷胺、塞來昔布對(duì)胃癌細(xì)胞株BGC一823的抑制作用TheinhibitoryeffectofProglumideandcelecoxibonhumangrasticcancercelllineBGC—823AbstractObjective：ToInvestigate

2、theinhibitoryeffectandmechanisisofcombinedtreatmentofthegastrinreceptorantagonistproglumideandtheselectiveCOX一2inhibitorcelecoxibonculturedhumangrasticcancercelllineBGC823invitroMethods：ThehumangastriccancercelllineBGC82

3、3wastreatedwithproglumideandcelecoxibrespectively，orincombinationThegrowthofBGC一823cellWasdetectedbyMTTassay；theexpressionsofCOX2and15一PGDHmRNAWasdeterminedbyrealtimequantitativereversetranscriptionpolymerasechainreactio

4、n(RTPCR)；theexpressionsofCOX2and15—PGDHproteinwasdetectedbyWesternblotting；thecontentofPGE2intheBGC一823cellculturefluidwasdetectedbyELISAResults：ProglumideandcelecoxibinhibitedthegrowthofBGC823cellsinatimeanddosedependen

5、tmanner(24—72h；Proglumide：2mmol／L，4mmol／L，6mmol／L，8mmol／L，10mmol／L；celecoxib：10I，tmol／L，301amol／L，50ttmol／L，801xmol／L，1001tmol／L)Proglumide(6mmol／L)andcelecoxib(50I，tmol／L)inhibitedtheproliferationofBGC823cellsatratesof4

6、11％and326％，respectivelyat48hProglumideandcelecoxibincombinationresultedinasynergisticeffectoninhibitinggrowth，theinhibitionratiois651％ProglumideandcelecoxibdownregulatedtheexpressionlevelsofCOX2mRNAandproteinofBGC一823cel

7、ls，respectively，buttheircombinationdownregulateditmoresignificantlythaneitheroftheagentsdid(Po05)whileProglumideandcelecoxibup—regulatedtheexpressionlevelsof15一PGDHmRNAandproteinofBGC一823cells，respectively，thetreatmentwi

8、thcombinationgroupwassignificantlyhiperthanthoseoftheBGC一823cellstreatedbythetwoagentsalone(PO05)theseparatedruggroupandtheuniteddruggroupcomparingtonormalcontrolgroup，isstatisticallysignificant(P001)Proglumideandcelecox