促紅細(xì)胞生成素（EPO）對類缺血神經(jīng)元的保護作用.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文促紅細(xì)胞生成素（EPO）對類缺血神經(jīng)元的保護作用姓名：楊慧申請學(xué)位級別：碩士專業(yè)：神經(jīng)病學(xué)指導(dǎo)教師：劉瑞珍20070507山西醫(yī)科大學(xué)頸士學(xué)位論文NeuroprotectiveeffectsofEPOonpfimaffculturedrat’SneuronsfollowingischemiclikeconditioningAbstractObjective：Toinvestigatethedamageofisch

2、emic—likeontheprimaryculturedrat7Sneurons，theprotectiveeffectsofEPOandtheoptimaldosageMethods：Corticalneuronsculturedfor7dayswererandomlydividedintofivegroups：controlgroup。ischemiclikegroupandplusthreekindsofconcentratio

3、nofEPOgroupstreatedThenmorphologyofneuronswasobservedunderinvertedmicroscopeThesurvivalratesofneuronsatdifferentgroupsweremeasuredwithMTTreductionassayandLDHexaminationTheapoptosisofneuronswasdetectedusingflowcytometrySt

4、atisticalanalysiswereusingSPSS120softwareStatisticalanalysisfixedthatP005issignificantResults：Weestablishedaexperimentedculturedrat’ScorticalneuronsbyusingthecommonculturemethodThecorticalneuronswasdetectedbyneuronalspec

5、ificenolase(NSE)immunocyto—chemicalstainingWeestablishedamodelbythemothedofischemiclikeconditioningonthecortialneuronsTherewasanotabledifferencebetweentheischemiclikegroupandthegroupsplusedEPO(PO05)Themediumconcentration

6、EPOcouldreduceapoptosisofneuronandincreasesurvivalratesofneuronButthelowerandthehi【ghconcentrationEPOgroupsexertlessactionalthoughhavingneuroprotectiveeffectsalsoConclusion：Throughtheflowcytometry，MTrreductionassayandLDH

7、examination，wefmdthatusingthemediumconcentrationEPOCanreducethedamageofneuronscausedbyischemic—likethroughreducingneuronapoptosisandincreasethesurvivalrateofneuronsWhatsuggeststhatEPOhasaneffectiveneuroprotectionKeywords