脫氮假單孢菌生產(chǎn)腺苷鈷胺提取工藝研究.pdf

1、天津大學碩士學位論文脫氮假單孢菌生產(chǎn)腺苷鈷胺提取工藝研究姓名：潘景峰申請學位級別：碩士專業(yè)：生物化工指導教師：聞建平20070601ABSTRACTVitaminB12iswidelyusedinmedicinestockbreedingandfoodadditive，whichisproducedmainlybyfermentationInthispaperanewmethodtodeterminevitaminB12inindust

2、rialfermentationwasstudiedVB12extractionfromfermentationbrothwasalsesearchedThestudyinthispaperwasbasedonindustyapplications，whichhavespecialvaluablereferencetoimprovementofVBl2fermentationandextraction1Methodtodetermine

3、VBl2infermentationbrothVitaminB12isproducedbyfermentationAdenosyleobalaminaremainlyproductsbyfermentationInthispaperVB12wasproducedbyPseudomonasdenitrificansfermentation，cellswerecollectedbycentrifugationSamplewasanalyze

4、dbyHPLCafterpretreatmentSamplecallbewellseparatedbythemethodin25rain1hedataallmeettheneedsofmethodologyThemethodissimple，rapidandaccurate，whichcanbeusedintheandextractionprocessofVitaminBl22FourmethodstoextractVBl2fromPs

5、eudomonasdenitrificansbycelldisruptionFourmethodsWeleappliedtodisruptcellforextractingVBl2，includingultrasonicationboiling，microwaveheating，highpressurehomogenizationThekineticsofcelldisruptionWaSstudiedExperimentshowedt

6、hatmethodsofboilingandmicrowaveheatingcallgethighextractionratioandproteinreleasedfromcellCallberemovedinoperatingprocessThemethodofboilingissuitableforindustryproduction，becauseitneedssimpleequipmentandcanbemanagedinlar

7、gescale一3SeparationandpurificationadenosylcobalaminfromfermentationbrothbymacroporousadsorptionresinsFourdifferentmacroporousadsorptionresinswerestudiedforresearchtheprocessesofstaticadsorptionanddesorptionforadenosylcob

8、alaminMeanwhileinfluenceofPHandtemperatureonadsorptionanddesorptionweredeterminedThetypeof3fresinhasexcellentperformanceinstaticadsorptionanddesorptionforadenosylcobalaminOptimaldynamicconditionswereinvestigatedfor3撐resi