魚肉中磺胺和孔雀石綠檢測方法研究.pdf

1、華南理工大學(xué)碩士學(xué)位論文魚肉中磺胺和孔雀石綠檢測方法研究姓名：李孟玻申請學(xué)位級別：碩士專業(yè)：食品工程指導(dǎo)教師：趙謀明何太喜20061001AbstractNowadaysoneofthemostnoticeableproblemsforfoodsecurityistheresiduesofveterinarydrugsinanimaloriginfoodstuffs，whichcausesmanyknownandpotentialarm

2、topublichealth。Themajorworkofthisstudyincludedthesimultaneousdetectionof5sulfonamidesdrags，malachitegreenanditsmetaboliteleacomalachitegreenresiduesinaquaticproductswithhighperformanceliquidchromatography(IIVtc3method。(1

3、)抽etelultsshowedthatthemethodofdeterminationofthosefivesulfonamidesresidulesasfollowing：Adddichloromethaneandmixsolventwithhi【ghspeedThencentrifuge3000f／rain。TransfersupernatantiIItoanewcentrifugetubeandaddnhexaneforextr

4、action。ThenvaporatetodrynessandconcentratebypassingWatersOasisHLB@solidphaseextacion(SPE)，Washwith5mlmethanolandtestbyHPLC。HFLCseparationwascarriedoutonaCloversflC18colum(250mmx46mm，i：d：5pro)withMethanolAceticacid：methan

5、ol：H20(1：10：89)asmobilephase，andColumntemperatureat38“C，Samplesize紐20／danddetectionwavelengthat265mn。(2)ThelinearrangeoffivesulfonamidesWC／eO～5伽魄皿underobeveconditions。CorretationcoefficientofSMR，SMT，SMM，SDM，SQXis0。9999，0

6、。9993，0。9997、09999、09993ThedeteetionlionlimitislO／Mg／kgWhenaddedis20～200Fg／kgTherecoverieswereintherangeof70～110％withRSDrangedfrom53to14％0)Therelultsshowedthatthemethodofdeterminationofmalachitegreenanditsmetaboliteleuco

7、malachitegreenresiduesasfollowing：Thesampleswereextractedwithacetonitrileacetatebuffermixture，andpartitionedintodichloromethane。Finalcleanuponanhydroussodiumsulphateandacidityaluminacolumn。TheanalyteswereseparatedbyHPLCw

8、ithdiodearraydetectorandquantifiedUSingexternalstandardcalibrationCUIVes。TheresiduelevelsaredeterminedbyusingCNcolumn，undervisibledetectionat618nm。(4)Thelinearrangeofmalachitegreenanditsmetaboliteleucomalachitegreenwere5