辛伐他汀預(yù)處理對大鼠腦缺血再灌注損傷Bcl-2、Bax表達的影響.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文辛伐他汀預(yù)處理對大鼠腦缺血再灌注損傷Bcl2、Bax表達的影響姓名：張林明申請學(xué)位級別：碩士專業(yè)：神經(jīng)病學(xué)指導(dǎo)教師：馬靜萍20100510山兩醫(yī)科大學(xué)七年制臨床醫(yī)學(xué)碩上專業(yè)學(xué)位論文EffectsofSimVastatinPreconditioningonExpressionofBcl2andBaxinRatswithFocalCerebralIschemiaReperfhsionAbstractobjectiVe

2、T0iIlVestigatetheeffectsofsiIIlVastatinpreconditioningonapoptosis鋤dexpressionofBcl2觚dBa】【inratsfollowingfbcalcerebmlischemiaandreperfusion柚dtoexploreprotectiVemechallismofsimVastatin0ntheneuronS，aIldproVidinganewtheoreti

3、calbasisfordinicalapplicationMethodsfony—ei曲tadulthealthymaleSDratswererandomlydividedintotheControlgroup，sh鋤operationgroup，№lCefebmlIschemiaReperfusiongroup(FCIR)，simV舔tatinpreconditioninggroup(SSpreConditioIlin曲Beforem

4、akingamodel，theratsinthesiInVastatinpreconditioninggroupwerclaVagedwithsimVastatiIl20mg／l【g，onceadayfor10conseclltiVedays，孤dthesameV01umeofnomalsalinewaslavagedfor10ConsecutiVedaysinthesh鋤opemtiOnandFCIRgroupsThemiddlece

5、rebralartery0cclusionreperfusionmodelwasmadebythesuturemethod(ischemiafor2hours，andreper凡sionfor4hours)Afterthat，theanimalswereneurolo西callyassessedona5Longapointscale，肌dthenbeheadedTheVolumeofinf打dionweremeasufedbyTrCst

6、aining，theexpression0fBcl一2卸dBaxweredetectedbyimmunohistochemistry，andthenumberoftheneuronapoptosiswasdetectedbyTUNELlksuIts1Thecerebralischemiarepemlsion伊oup鋤dsimVastatillprecongditioning黟oupaUhaVedi臟remneurolo西calimpai

7、咖ent世erCerebralischemiarepemlsionComparedwiththecerebralischemiareperfusiongroup，thenuemlo舀calscoresofthesimVastatinpreC0nditioninggroupsreducedobViously，thetwogroupswerestatisticallysignificaIlt(PO05)2AnerTTCstainingthe

8、wholebrainsOfthecontrolgroup觚dtheshamgroupwercred，theri曲tf如ntal，parietalC0nicalandstriatumofthebrainsofthecerebralischemiareperfusiongroup孤dsimVastatinprecongditioninggrouparewhiteinfarctsComparedwiththecerebralischemicr

9、eperfusion黟oup，whiteinfarctsareaofthesimVastatinprecongditioning伊oupdecreased，theresultswerestatisticaUysignificant(P001)3Comparedwiththecontrolgroupandtheshamgroup，theexpressionofBcl2andBaxofthecerebralischemiarepe—usio

10、n黟oupandthesimVastatinpfecon酣itioning野oupareincreased(PO01)，CompaIedwiththecerebralischemiareperfnsiongroup，theexpressionofBcl2ofthesimVastatinprecongditioninggroupishigher(P005)，theexpressionofBaxislower(P005)whileapopt

11、oticcellsrarelywereseeninthecontrol舒oupandthesham黟oup，apoptoticcellsofthecerebralischemiarepe—hsion留OupandthesimVastatinprecongditiOning伊0upincreasedobViously；Comparedwiththecerebralischemiarepedhsiongroup，thesimVastatin