腦缺血后自主神經(jīng)功能障礙對(duì)心血管系統(tǒng)的影響.pdf

1、摘要摘要目的:本實(shí)驗(yàn)通過(guò)連續(xù)記錄大鼠局灶性腦缺血再灌注不同時(shí)間段的動(dòng)脈血壓(arterialbloodpressureABP)、心電圖(electrocardio鄉(xiāng)amECG)信號(hào)、心率(heartrateHR)，以及腎交感神經(jīng)活動(dòng)(RenalSympatheticNerveActivityRSNA)并分別進(jìn)行處理和分析，探討急性局灶性腦缺血后自主神經(jīng)功能障礙對(duì)心血管系統(tǒng)的影響。方法:采用線栓法制作大鼠局灶性腦缺血再灌注模型，將30只成

2、年雄性SD大鼠隨機(jī)分為缺血組(ischemian=24)及假手術(shù)組(shamoperationn=6).缺血組再根據(jù)缺血時(shí)間不同和是否再灌注又分為4個(gè)組，分別為:缺血1小時(shí)組(ischemiaforlhour11)、缺血3小時(shí)組(ischemiafor3hours13)、缺血5小時(shí)組(ischemiafor5hours15)和缺血3小時(shí)再灌注2小時(shí)組(ischemiafor3hoursandreperfusedfor2hours1382

3、)，每組6只大鼠。通過(guò)PowerLab8spSCSIUSB記錄儀連續(xù)采集ABPHRECG和RSNA信號(hào)。從記錄的ABPHRECG以及RSNA時(shí)間序列中，以每2分鐘為一個(gè)時(shí)間單位，利用Chart4軟件獲得平均動(dòng)脈壓、心率、心率變異性序列及腎交感神經(jīng)活動(dòng)。對(duì)經(jīng)過(guò)等間距處理后的心率變異性序列進(jìn)行功率譜分析。對(duì)實(shí)驗(yàn)組的所有數(shù)據(jù)進(jìn)行配對(duì)t檢驗(yàn)。結(jié)果:1MABP與HR:缺血組與假手術(shù)組相比，缺血后均顯著升高M(jìn)O.05)缺血后30分鐘MABP達(dá)到峰值

4、，而缺血2.5小時(shí)HR到達(dá)高峰并且維持明顯高于缺血前的水平再灌注后MABP與HR均有不同程度的降低，MABP比缺血前水平低而HR比缺血前水平高。2HRV:急性腦缺血后，總功率譜(totalpowerTP)、低頻段功率(lowfrequencypowerLF)和高頻段功率(highfrequencypowerIIF)均明顯降低(P0.05)，中頻段功率(mediumfrequencypowerMF)及MFHF比值均明顯升高(P0.05)，

5、缺血3小時(shí)以后，各項(xiàng)HRV頻域分析指標(biāo)均明顯下降(低于基AbstractAbstractObjectives:ToinvestigatethechangesinautonomicnervoussystemcausedbyfocalcerebralischemiainSDrats.Arterialbloodpressure(ABP)electrocardiogram(ECG)heartrate(HR)andrenalsympathetic

6、nerveactivity(RSNA)wererecordeddealtwithandanalysed.Methods:FocalcerebralischemiareperfusioninSDratswasinducedbyintraluminalocclusionofmiddlecerebralartery.Ratswererandomlydividedintothefollowinggroups:pseudosurgical11(i

7、schemiafor1hour)13(ischemiafor3hours)15(ischemiafor5hours)and1382(ischemiafor3hoursandreperfusion.(for2hour.Therewere6ratsforeachgroup.Ratsinexperimentalgroupsweresuferedfocalbrainischemiareperfusionthroughmiddlecerebral

8、arteryocclusionbythenylonthread.ABPECGSHRandRSNAwerecollectedfordisplayingand.lateranalyzing勿PowerLab8spSCSIUSBdataacquisitionsystem(ADInstruments).UsingthesoftwarenamedChart4meanarterialbloodpressure(IvIABP)meanheartrat

9、e(HR)andsequenceoftheinterrelateddataofheartratevariability(HRV)wereobtainedfromthetimeseriesin2minutesperiod.TheHRVsequenceswereequalizedandprocessedbypowerspectralanalysisthroughtheFastFourierTransform(FFT).Pairedttest

10、swereperformedforalldataofgroups.Afteraprocessorfortissuemanagethebrainswerecorona)sectionedat30tmbyarotarymicrotomeandstainedwithHematoxylinandEosintoobservethemorphologicalalterationofneuroninischemicarea.Theotherbrain

11、swerecorona)sectioneddirectlyat2mmandstainedwithTTCtoobservethealterationinischemiaarea.Results:1.MABPandHRinsurgicalgroupswereincreasedafterischemiavscontrol.MABPreachedthehighestlevelinhalfanhourafterocclusionandHRreac

12、heditsclimaxin2.5hoursafterischemiamaintainedatahigherlevelthanthatofthepreischemia.AfterreperfusionMABPandHRweredecreasedtodiferentdegrees.MABPwasrecoveredtothepreischemialevelbutHRwaschangedtoalittlebithigherthanthat一I