異常表達(dá)基因的檢測.pdf

1、山東大學(xué)碩士學(xué)位論文異常表達(dá)基因的檢測姓名：劉煒申請學(xué)位級別：碩士專業(yè)：金融數(shù)學(xué)與金融工程指導(dǎo)教師：欒貽會20090430山東大學(xué)碩士學(xué)位論文DETECTIoNoFDIFFERENTIALLyEXPRESSEDGENESLiu，Wei(SchoolofMathematics，ShandongUniv，jinan，250100，Shandong)ABSTRACTAcentralaimincancerresearchistoidentify

2、alteredgenes(overordownexpressedgenes)thatplayacausalroleincancerdevelopmentmRNAmicroarrayexperimentsprovideafastandsystematicwaytoidentifydiseasemarkersrelevanttoclinicalcareAcommonexperimentaldesignisthecomparisonoftwo

3、setsofsamplesfromdifferentphenotypes(diseasesandnormaltissue)，withthegoalofsearchingforgenesshowingdifferentialexpressionThisisusuallydoneviastatisticaltestingproceduresand，often，subsequentmultipletestingcorrectionsAllth

4、esemethodsuseaonegeneat—atimestrategyconsideringonlytheassociationbetweensinglegenesandthephenotypeTheidentificationofdifferentiallyexpressedgenesisaquestionwhicharisesinabroadrangeofmicroarrayexperimentsManymethodshaveb

5、eenproposedtodetectdifferentiallyexpressedgenesDudoitandotherscommonlyusedmethodAmongthem，tstatisticsisthemostInthemajorityofcancertypes，heterogeneouspatternsofoncogeneactivationhavebeenobserved；thus，traditionalanalytica

6、lmethodsthatsearchforcommonactivationofgenesacrossaclassofcancersamples(eg，ttest)willfailtofindsuchoncogeneexpressionprofilesInstead，amethodthatsearchesformarkedoverexpressioninasubsetofcasesisneededRecentlyTomlinsaadoth

7、ers【414haveproposedthe”canceroutlierprofileanalysis”(COPA)methodfordetectingcancergeneswhichshowincreasedexpressionsinasubsetofdiseasesamp!esMorerecentlyTibshiraniandHastie【1】proposedtheoutliersum(OS)statistictodetectcan