GnRH多肽免疫去勢(shì)疫苗在大鼠體內(nèi)的殘留及免疫去勢(shì)動(dòng)物肉品安全的研究.pdf

1、四川農(nóng)業(yè)大學(xué)碩士學(xué)位論文GnRH多肽免疫去勢(shì)疫苗在大鼠體內(nèi)的殘留及免疫去勢(shì)動(dòng)物肉品安全的研究姓名：王霜申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物物理學(xué)指導(dǎo)教師：曾憲垠劉顯義20070501SummaryInlate1960s，HormoneiinnluRo—nutralizationtechniquesemergedonthefoundationofimmunologybiochemistryandendocrinologyHormoneimmuno

2、nutralizationtechniquesofferedanewalternativetotraditionalsurgicalcastrationinanimalproductionGonadotrophinreleasinghormone(GnRH)isreleasedfromthebasalhypothalamusmedianeminenceandstimulatestheanteriorpituitaryglandtosec

3、reteluteinisinghormone(LH)andfolliclestimulatinghormone佰SH)whichtogetherregulatetesticularandovarianfunctionsImmunizationagainstthehormonepreventscirculatingGnRHfrombindingtopituitaryreceptorsthroughimmunoneuWalization(i

4、ebindingbyantibodies)，henceblockingfurtherinteractionandphysiologicaleventsStudiesontheavtiveimmunizationagainstCmRHwerecarriedoutinanimalssuch勰pigs，catllcs，goatsandcMkemAndmuchprogre鸛wereobtainedHowevercustomersasked：wh

5、etherthereisresidueofthevaccineintheanimalsHowaboutthemeatsafetyoftheimmunizedanimalsAsfaxasweknowmoreported011theresidueofthevaccineintheanimalsandthemeatsafetyoftheimmunizedanimalswasfoundInordertoanswerthosequestionst

6、heresidueofthevaccineintheanimalsandthemeatsafetyoftheimmunizedanimalswffteinvestigatedincurrentstudy，theresultsobtainedarcasfollows：1RadioiodinelabelingofGnRHTandemDimer(GnRHTDK)ThetwophasecMoramineTmethodwasuesdinthera

7、dioiodinelabetingofGnRHTDKTheiodineincorporationwasdeterminedbytrichlomaceticacidfrCA)anda7mysurveymeterThehbeledcompoundWaspurifiedbyulWafiltrationThepurityofthelabeledCmRIITDKWasdeterminedbyTCAandtheimmunologicactivity

8、invivowasdeterminedTheresdtsdemonstratedthattheiodineincorporationradioehemicalpurityandspecificradioactivityof125IGnRHTDKWas786906，9126％and194105Bqpg1respectivelyandtheimmunologicalactivityof125IGnRHTDKwasnotaffectedbyt

9、heradiolabeling2ResidueoflzsIGnRHTDKOVAinratsTissuedistributionandexcretionofGnIUI—TDK0vAinratswereinvestigatedTheresultsdemonstratedthat24daysafterimmunizationtracequantityOnthelevelof045士023I_tg／gWasfoundintheinjection