多發(fā)性骨髓瘤患者和正常人DAZAP2基因的差異表達(dá)研究.pdf

1、中南大學(xué)碩士學(xué)位論文多發(fā)性骨髓瘤患者和正常人DAZAP2基因的差異表達(dá)研究姓名：沈熔申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：胡維新20050401碩士學(xué)位論文摘要ABSTRACTInordertofindnewmultiplemyelomaassociatedgene，Yi—WuShi，etalanalyzeddifferentialscreeningofcDNAchipsbetweenmRNAfrombonemarrow

2、mononuclearcellsofMMpatientsandnolTnalcontrols，andfoundthatD氏z姆2wasamostprofoundlydownregulatedgene(Cy5(MM)／Cy3(normal)=0、091)0bjectiveDetecttheexpressiondifferenceofDAZAP2betweenⅢpatientsandnormalcontrolsonRNAandproteml

3、evelMethods(1)DetectexpressionofDAZAP2mthebonemarrowmononuclearcellsofMMpatientsandnormalcontrolsbymethodsofsemiquantitativeImPCRandWesternblotting(2)AnalyzetheexpressionofDAZAP2ofMMpatientsandnormalcontrolsbychisquarete

4、standseekifitalsohascorrelalionwiththeclinicaldataofⅢpatientsResultsAmplifiedproductofDAZAP2byRTPCRisasegmentof400bp9MMpatientsexpresseditandotherllpatientsdidnot、v11ile12normalcontrolstamedoutallexpressitResultsofWester

5、nblottingrevealedthat9specimensofMMpatientshadthe17KDDAZAP2protein，andllcasesdidnot12normalcontrolshaditasexpectedConclusion(11TheexpressiondiffefenceofDAZAP2betweenMMpatientsandnormalcontrolsisprominent(p001)(2)Itcannot

6、beinducedthereiSacorrelationamongstDAZAP2expressionandthegenderand／ortheageofMMpatients，andweneedmoresamplesfortheassayTheexpressionofDAZAP2mightbediagnostictoMMKe、3vordsmultiplemyelomadmⅥ1regulated，MYDOG1／DAZAP2，tumorsu