斗南蘋果自交不親和基因型的研究.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文斗南蘋果自交不親和基因型的研究姓名：陳超申請學(xué)位級別：碩士專業(yè)：森林培育學(xué)指導(dǎo)教師：李保國齊國輝20050619StudyoRselfincompatibilitygenotypeofDounanapplecultivarName：曲enChaoSpeciality：ForestSilvicultureSupervior：LiBaoguoQiGuo—huiAbstrac毫AppleisakindofGametop

2、hyticselfincompatiblefruittree，SOitisnecessarytoarrangevarietiesforpollinationorperformartificialpollinationforstableyield。Pollinationcompatibilityamongcuttivarsmustbeconsideredfirstbeforearrangingpollendonor，andpollinat

3、ioncompatibilityisdeterminedbySgenotypeofthecultivars，whentheirSgenotypesarethesame，thenexhibitionsincompatible，butwhentheirSgenotypesaredifferent，andexhibitionscompatible。Dounanisanewapplecultivar，butitsSgenotypeisunkno

4、wn，Inthislhesis，SgenotypeofDounanwasstudied，SOastoprovidetheorybasisforappleproductionTheresultswereasfollows1Thepollengerminationpercentageofthecultivarswereupto7333％，SOallofthemcouldbeusedaspollination，Theamountofpolle

5、ntubesfromstylebottomsandpercentageofstylewithpollentubesthroughtheirbottomsinsemivitrocultureofDounanstylespollinatedwithGoldenDeliciouswhichshowedcross—incompatiblewas0，58and29。03％，buttheywere3064。70and7742％一8667％whenD

6、ounanstyleswerepollinatedwithFuji，InduandBaskatongwhichshowedcross—compatible，TheresultofpollinatedstylesinsemivitrocultureshowedthatDounancontainedS2orS3一allele2TheOD26dOD2s0ratiosofdi濂rentcultivarsDNAdistilledwithimpro

7、vedCTABmethodafterpurifiedwere1819，whendetectedwithagarosegeleletrophoresis，DNAshowedatrimbrightbandwithoutsmear，andtherewasnesiduebrightinsamplehole，TheresultshowedthattheDNApuritywashigh，andwithoutdegradation，itwassuit

8、ableforPCRRFLPanalysis3TheoptimizationsystemofSapplegenePCRamplificationwasasfollows，In20LreactionsystemtheamountofTaqDNApolymerasewas1U，dNTPsamountwasO25trmjol，L～。primeramountwas04_tmolL～，M92concentrationwas25mmol￡‘l，te