電針對(duì)獼猴腦缺血的效應(yīng)及對(duì)胰島素樣生長(zhǎng)因子-1的作用.pdf

1、復(fù)旦大學(xué)博士學(xué)位論文電針對(duì)獼猴腦缺血的效應(yīng)及對(duì)胰島素樣生長(zhǎng)因子1的作用姓名：高煥民申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：中西醫(yī)結(jié)合基礎(chǔ)指導(dǎo)教師：程介士2002.4.18電針對(duì)肆鬟腦缺血的效應(yīng)爰時(shí)腱島素樣生長(zhǎng)因子1的作用英文摘要ABS7rRACT：EffectsofElectroacupunctureonFocaICerebralIsehemiaandExpressionofInsulinlikeGrowthFactorIinMonkeyClinica

2、lpracticeshaveshownthatacupuncturecanpromotetheneurologicalrecoveryinpatientswithischemicstrokeAlsolaboratoryinvestigationsshowthatelectroaeupuncture(EA)hasthenettroprotectiveeffectsoncerebralischemiaHowevertherearegreat

3、differencesbetweenratsandhuman。thereforeitsdifficulttointerpretthehumanstrokefromtheratmodelsFurthermoremestrokepatientsCannotbetreatedbvEAmerelyResultsofEAtreatmentcombinedwithmedicine疳Dmdiversitystrokepatientsmightbeve

4、rycomplicatedThusthemonkeymodelofthemiddlecerebralarteryocclusion(MCAo、shouldbeusedinEAstudyCerebralischemiaproducescelldamagewhileprovokingthebodyselfprotectivemechanismAmongtheneurotrophicagents，insulin—likegrowthfacto

5、r1(IGF1)，hasbeenreportedasoneofthemostpowerfulprotectivefactorincerebralischemiaHoweveritisunknownwhetherIGF1iSinvolvedinEAeriectagainstcerebraljschemiaDNAchipsdetectingmanygenesprovideaplatformatthesalneconditionTherefo

6、reIGF1mRNAwasanalyzedwithDNAchipsandconfnmedbyinsituhybridizationandimmunohistochemistryTheresultsindicatedthatthemonkeyreversibleMCA0modelproducedbyinsertingaselfmadeballooncatheterintotheproximaloftheMCAcouldproduceamo

7、delmimickingthehumanfocalischemicstrokeconfmnedbyneurologicalevaluation，EEG，neuropathologicalevaluationand閻SCanEAcouldincreasethe10ealcerebralbloodflowatthestriatum，promotetherecoveryofEEG，attenuatebrainedema，decreasethe

8、infarctareaincreasetheresidualcells，up—regulatetheexpressionofIGF1mRNAandproteinwhichdecreasedbytheischemiaiIlstriatumTheseresultsimplied也atEAiseffectivetoextenuatecerebralischemicinjuriesfollowingthereversibleMCAOinmonk