31165.蘇云金芽孢桿菌cry基因型的鑒定

1、河北大學碩士學位論文蘇云金芽孢桿菌cry基因型的鑒定姓名：胡曉婷申請學位級別：碩士專業(yè)：動物學指導教師：馮書亮20090501AbstractAbstractInordertosearchthevaluableresourceofBacillusthuringiensis(Bt)inChinaandfindnewandhightoxincrygene，eighthundredsstrainsofBacillusthuringiensis

2、wereisolatedfromsomedifferentsoil—typesamples。RegulationofdistributionofBtstrainswassummarized：thezoneswhichhadmorevegetationandSetterprotectedoriginalvegetationhadmoreBtstrains；themorehumussoilsampleswereincludedinthemo

3、reBtstrainswerecontainedMostshapesoftheBtisolatescrystalswereround，meanwhile，theshapesofcrystalsincludingbipyramidalandsquare，severalstrainscontainscoexistentlytwoorthreekindsshapesofcrystals。ItwasshownthatChinahadabunda

4、nceresourceofBtstrainsThecrygenetypesof800BtisolateswereidentifiedbyuseofPeRRFLP(RestrictionFragmentLengthPolymorphism，RFLP)systemWedesignedsomeuniversaloligonucleotideprimerswhichwereusedinthePeRsystemThePeRamplifiedpro

5、ductsweredigested、析thoneortworestrictiveendonuclease，andthecrygenetypesinthestrainswereidentifiedonthebasisoftherestrictionfragmentlengthpolymorphismWecametoaconclusionthat107●●isolateshadcrylgenes，202isolateshadcry2gene

6、s，I5isolateshadcry3genes，361isolateshadcr))8genes，1isolateshadcry7genes；57isolateshadcr)9genes，1isolatehadcry40gene，2isolateshadcry6genesand2isolateshadcryllgenesThecrygene—tapesof58strainshaven’tbeenascertained，andtheym

7、ayhaveexcludecryl35andcry40crygenetypesornewcrygenetypesWedetectedthreestrainshadcryl、cry2，cry8andcry9genetypescombinationforthefirsttimeTheirshapesofthecrystalswerediamondandthestrainsexpress130KDaand90kDaproteinsWhethe

8、rtheyhadextensivepesticidalrange，weneededtoresearchinthefutureOne1386bpfragmentofthecry40genewasgainedfromtheFSQ87stainandWasclonedandsequencedIthad88％sequencehomologywiththecry40BaholotypegeneThegenemaybeanewthethirdgra