27297.石油烴對(duì)馬糞海膽dna甲基化與補(bǔ)體c3的毒理效應(yīng)研究

1、大連海事大學(xué)碩士學(xué)位論文石油烴對(duì)馬糞海膽DNA甲基化與補(bǔ)體C3的毒理效應(yīng)研究姓名：劉美華申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：環(huán)境科學(xué)與工程指導(dǎo)教師：熊德琪20090601ABSTRACT上Jetrole啪hydrocarbonsisoneofthemain‘pollutantsinmatine鋤virollIllentInmlsp印er，seaurchin(HemicentrotusPulcherrimus)wereselectedaStestor

2、ganism乜xpenme刪researchontheseaurchingenomewideDNAmethylationlevelandtheprobablIityofcomplementC3genemutationpollutedwiththepetr01e啪bydroc甜怕ns1nvestigatingthetoxiceffectsofpetroleumhydrocarbonsonthemarineorg；枷smsinm01ecul

3、arleVelAt1ast，probingthetoxicitymechanismofpetr01eumhydrocarbonpollutiononaquaticorganismsSoitCanprovidereferencetoearlyw踟i119a11driSkassessmemforpetroleumhydrocarbonpollutiononthem撕neenviroI瑚entAnalyzingcheIIlicalsubsta

4、ncesOnbiologicalDNAmethylationisa11import鋤moleculartoxlcologYme觚stoassessthelevelofitsbiologicaltoxicityInthispap％j119n巾er內(nèi)mlallceliquidchromatographyisusedtoanalyzetheseaurcKnDNAmethylatlonchangeswhichexposedtodifferent

5、concentrationsandtimeofNo0dlsperseddieseloilTheexperimentsresultshowsthatpetroleurnhydrocarbonscaIlcauseseaurcllingenomechangesintheoveralllevelofmethylation，晰ththeextensionotexposuretimeⅡ1erewouldbealowmethylationphenom

6、enon，butwehaven’tfounds1舯11c鋤lydose。responserelationship，whichindicatethatpe仃0le啪hydroc砷onscanimpactseaurchinDNAmethylationanditsregulationinvivo1heseaurchincomplementsystemisaIlimportantfunctionthatreSponsebodv1‘砌unec印∞

7、nyInthispaperInordertoexplorethatifthe5methylcytosineis”notspotstfofmutationgeneticindicatorscomplementC3genem似溉吣觚herstudledontheDasisofthestudyofindicatorsofseaurchinepigeneticDNAmethvlationexposuretolowconcentrationpet

8、roleumhydrocarbonsThetestresuItsfoundthatonepnmers(pnmersB6)ofthePCRproductexistthep01ymorpllism，1184occurredinmeco州erSlonofCTmutation，thatmnlirloacidfromthreoninetoisoleucineAnditwasalsotoulldastheconcentrationofpetrole

9、umandexposuretimeincreaSed，theprobabilityofmutationofseaurchincomplementC3geneincreasedtooItis1ndlcatedthatpetroleumhydrocarbonpollutioncancauseDNAmutationsinseaurcllin[hispaperanalyzetheDNAmethylationcontentandcomplemen