30092.從加納籽中提取、分離純化5羥基色氨酸的工藝研究

1、西北大學碩士學位論文從加納籽中提取、分離純化5羥基色氨酸的工藝研究姓名：聶利芳申請學位級別：碩士專業(yè)：細胞生物學指導教師：李多偉20090610Extraction，SeparationandPurificationof5一hydroxytro—phanfromGriffoniasimplicifoliaAbstract5hydroxytrophan，whichhasmanybiologicalfunctions，isallimport

2、antmentaldrugandcarlbeusedasrawmaterialstomakehealthprotectingmedicineItiswidelyusedinclinicaltreatmentAndtherewillbeabrightmarketprospectduetoitsgreatvalueTherefore，itisofgreatusetostrengthentheresearchinextraction，sepa

3、rationpurificationanddetectionof5hydroxytrophamAfricangrainGriffoniasimplicifoliacontains5hydroxytrophanSoweuseGriffoniasimplicifoliaasrawmaterialstostudy5hydroxytrophanTheproductionprocessof5一hydroxytrophanfromnaturalpl

4、antsisoptimizedTheresearchinextractionseparation，purificationanddetectionmethodsiscarriedintheexperimentwiththeaimoflowercostTheachievementsare邪follows：1Theorthogonaldesignoffourfactorsandthreelevelsisusedtoselecttheopti

5、malextractionmethodbasedonthesinglefactorexperimentsandresearchinextractionmethodsandextractionsolventThebestextractionprocess：Griffoniasimplicifoliadegreasedpowderthrough20meshsieve；temperatureis80℃：theextractiontimesal

6、etwo；theratioofmaterialtoliquidis1：15；theextractiontimeis3h2Anewpurificationprocessof5hNroxytrophanhasbeendevelopedinthisworkThemainstepsarOcontainedinthisprocedure，ie，clarificationofwaterextractof5hydroxytrophan，macropo

7、rousadsorptionresinchromatographyactivecarbondccoloufiz。ationcrystallizationandrecrystallizationTheoptimalconditionisdeterminedTheresultsshowthattheyieldis665％，extractionyieldis5938％andthepurityof5一hydroxytrophanishighto

8、9730％3Thinlayerchromatography(TLC)methodisemployedtomakequalitativeandquantitativeanalysisThedeveloperisnormalbutanol，glacialaceticacidandwaterwiththeratio3：1：1Visualizationreagentis02％ninhyd血ethanolsolutionRfis055Scanni