黃單胞菌L4毒素抑制雜草的作用機理研究.pdf

1、浙江大學碩士學位論文黃單胞菌L4毒素抑制雜草的作用機理研究姓名：張自玲申請學位級別：碩士專業(yè)：遺傳學指導教師：李永泉20030601AbstractThechemicaIandphysicaIchamctersofxallthomon私L4phytotoxin，including池heatst曲川cyliposolubili吼molecularweight鋤de腩ctofpHonweedcon”olactiVi吼thenitsactio

2、nmechanismont11ecelllevelw船explored，andtheinhibitoryconditiOnOf爿如腩omoH∞L4phytotoxinw踮alsofunherstudiedResearchesonbacterialherbicidearemostlylimitedtoweedcontmltestwithph”otoxinf如mbacterialfe冊entationbrothHoweVer，mechall

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4、mD”卯L4fcmentationbrothdemons衄tedtheL4phytotoxinwasvery1ipos01ublecompounda11dbelongingtomicromolecular，f叭herexperimentsindicateditwasverystableunderhi曲temperaturc鋤dph”otoxinhaVehi曲estbioactivityatpH411leexperimentofselec

5、tingadjv鋤tshowedtllat1ween_80，JFC，SDS，Siliconcw硒suitablefor朐阿腩伽D療甜L4ph”otoxinatlowerconcentrationand1％TWeen80、vasbestadjVantAner彳md陽療腩w旭f№，弛r(redrootamarant)wereinfectedbyx州曲D卅DH礎￡一phytotoxiningreemlouse，thevariationofso

6、lubleprotein，DNA，peroxidase(POD)actiVity，malondialdehyde(MDA)，photosyntlleticconVersionemciency，ceIlmemebranepenneability鋤drespiratoryratioofleaveswerederIeminedwimCoomassieBrilliaIltBlue，UVspectmscopy，guaiacol，ttliobafb

7、ituricacid，P：AM20l—pulsemodulationfluorometer，conductometryandL1—6400PortablePhotosynthcticSystem，孤dttlech鋤geofcellultr∞t九lcture舭d印ide珈Aisofleaveswerealsoinspected謝t11mmsmissionelectronmicroscope(TE】Ⅵ)andsca衄ingelectmnmi

8、croscope(SE岣Theexpe曲lcntresultsshowedrespiratoryratio趴dPODactivityincre船ed106％肌d50％，respectiVelyaftertrcat2h，photosyntheticconversionemciency’MDAandsolubleproteinw船notinhibitedUltmstmctreobservationrevealcdthat也ethylal【o