四個(gè)重要和瀕危畜禽細(xì)胞庫(kù)的構(gòu)建及其生物學(xué)分析.pdf

1、江西農(nóng)業(yè)大學(xué)碩士學(xué)位論文四個(gè)重要和瀕危畜禽細(xì)胞庫(kù)的構(gòu)建及其生物學(xué)分析姓名：丁鴻申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：劉海生關(guān)偉軍2003.6.1ConstructionandbiologicalanalysisoffourimportantandendangereddomesticanimalcollbankAbstractSmallTailHansheepearskintissue，Beijingfattingchickenan

2、dJingxingyellowchickenembryos(9～13days)andBeijingduckembryos(13～15days)werecultivatedwithdirectadherent——cultivationmethodstogetfibroblastsomaticcells，thentheywerefrozeninliquidnitrogensuccessfullyThecellswerepassagednom

3、orethan5generations。andthecellfreezingdensityisbetween3i06cells／mland5106cells／m1Thegrowthcurvesoffourkindsanimalappeared“S”shape，andillustratedthatthecellsgrowthhavethreephases(1atentphase，109arithmicmultiplicationphase

4、andlevelstaticphase)Theresultsofmeasuringthecellsurvivalrateshowedthatthecellsurvivalratewoulddescendaftercryopreservation，buttsurpassed85％TheanalysesofthechromosomalkaryotypesandG—bandindicatedthatthecellswerediploid，th

5、echromosomeaberrationswasunder2％，thusthehereditarvcharacterisstableBecauseofslightdamagestoexperimentalanimalandeaseofmanipulation，thismethodmaylaythefoundationofhereditaryresourcespreservationofChineseimportantandendang