玉米赤霉醇分子印跡聚合物的制備及其在殘留分析中的初步應(yīng)用.pdf

1、中國農(nóng)業(yè)大學(xué)碩士學(xué)位論文玉米赤霉醇分子印跡聚合物的制備及其在殘留分析中的初步應(yīng)用姓名：彭濤申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：李俊鎖扈洪波2002.6.1StudiesonSynthesisofAntizeranolMIPsandItsPreliminaryApplicationstesidueAnalysisPengTaoSupervisor：AssociateProfessorLiJunsuoandAssociateProf

2、essorHuHongboCollegeofveterinarymedicine，ChinaAgriculturalUniversityBeijing100094AbstractMolecularimprintingisbecomingincreasinglyrecognizedasapowerfultechniqueforthepreparationofsyntheticpolymerstailormaderecognitionsit

3、esforcertaintargetmoleculesMolecularlyimprintedpolymers(MIPs)haveadvantagesofhighaffinityhighseleetiv時(shí)forimprintmolecule，longstabilityandeasilysynthesisDuringthelastyears，theirapplicationtoseparationsofdrugs，environmenta

4、nalysis，catalysts，andsoon，arebeingactivelypursedInourresoarc】f，ZERMIPshavebeensynthesizedat600inmethanol，acetonitrile，chloroform，andtlleircompound，usingzeranol(ZER)asimprintmolecule，methacrylicacid(MAA)or4vinylpyridine(4

5、VP)asfunctionalmonomerandethyleneglycoldimethacrylate(EGDMA)ascross1inkerAffinitytestindicatesthatMIPssynthesizedinlesspolarsolventandsoIventawithgoodsolubilitytoimprintmoleculehavemuchhigheraffinityeapacityComparingwith

6、clenbuterol(Clen)MIPsnonspecialaffinityiSrgvealeddewtoionicinteractionbetweenMIPsandanalystsMIPssynthesizedbyMAAinchlorofclrmacetonitrile(51)areprovedmostefficientwithhighaffinitycapacityandlownonspeeiala街nityAdsorptionk

7、ineticstestdemonstratestheseMIPscangettoequilibriumin4hoursTheirdissociationconstantandmaximaladsorptionquantityarecalculatedbyScatchardequationKd=248x101mol／l，Qm“=O59x10～m01／gCross—reactivitystudycomparingwithivermectin

8、，Clenand3ZERstructurallyrelatedcompounds，showsspecialaffinityreliesonstructureandfunctionalgroupsofanalystsTheseMIPsshowgoodselectivitySolidphaseextraction(SPE)columnisachievedbyfillingZERMIPssorbentssynthesizedbvMAAinch

9、lorof01Tnacetonitrilef51)into2mLcartridgeInelutiontestpolarsolventisrevealedtohavestrongerelationabilitythanapolaronesTheoperationmethodofthisSPEcolumnhasbeenestablishedwithfollowingsteps：applicationwith1mLchlorofoFITIso