豬體細胞核移植及幾個相關(guān)問題的研究.pdf

1、東北農(nóng)業(yè)大學博士學位論文豬體細胞核移植及幾個相關(guān)問題的研究姓名：孫興參申請學位級別：博士專業(yè)：動物組織胚胎學指導(dǎo)教師：譚景和2001.4.1堡鲞墨墊蔓一——型型垡一StudiesonporcinesomaticnucleartranSferatldsomerelatedproblemsABSTRACTNucleartransfertecllniqueisimpon卸tnotonlyforthestudyofnIlclea卜c”叩lasm

2、interaction，butalsohasextensiveappIicationsinhum柚clinicsand徹imalh璐bandry1th鷂beenprovedthatporcinesomaticcJ0ningisthemOstdi茄cult啪ongallmammali鯽sp∞i銘In，thisexperime吣westudiedporcinec】0ningbytransp】an協(xié)tionofcumulllsce卸dcult

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9、pesGVchrom“nconngurationwercobservedwithHocest33342stainingintheporcineooc”esW脅t，lefoJ】jcuJardeveloPment，theproportionofGV1ooc”ew酗incre髂edooc”esf吣mthepreantralfolIicleswereallatGVOstageTheproportionofGV3ooc”ewaSincreased

10、withtimeduringjnVitromaturationNoGV一3stagew越Observed，butGV一2stagewaSfoundinvivoI824hoursaRerhCGiniectjOn7丁hediff毫rentGVchromatinconfiguratjonwasnotsynchroni恐dwhenAandBtypesofoocneswerepreculturedinabsenceofhomlonefor12ho