蒙古羊全基因組測序及基于轉錄組分析的抗寒性狀遺傳基礎研究.pdf

1、內蒙古農業(yè)大學研究生學位論文獨創(chuàng)聲明本人申明所呈交的學位論文是我本人在導師指導下進行的研究工作及取得的研究成果。據我所知，除了文中特別加以標注和致謝的地方外，論文中不包括其他人已經發(fā)表或撰寫過的研究成果，也不包括為獲得我?；蚱渌逃龣C構的學位或證書而使用過的材料，與我一同工作的同志對本研究所做的任何貢獻均已在論文中作了明確的說明并表示謝意。申請學位論文與資料若有不實之處，本人承擔一切相關責任。論文作者簽名：4交叢叢日期：’盤壘!塑=(!

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3、其他手段保存論文。論文作者簽名：《蠡毯盤厶一指導教師馘：2駐!奴日期：兇墼么!!oPreliminaryAssemblyofMongolianSheepGenomeandTranscriptomeAnalysesofcoldtolenceinMongoliansheepAbstractMongolianSheep，beingknowntOitshardinessandgoodmeatqualityistheancientsheepbre

4、edinChinaThegenomecontainsagreatdealofpreciousgeneticresourcesInthisstudywesequenedthewholegenomeoftheMongoliansheepusingIlluminaHiseq2000sequencingtechnologyplatformandthewhole—genomeshotgunsequencingapproach，inordertop

5、rovidethebaseoffilteringthegeneswithfinepropertiessuchasanticoldnessdiseaseresistanceere。Theresultshowedthatthegenomesizewasestimatedin29lGbAssemblycontigN50lengthiS201KbseaabldN50l朋gthiS27313KbAverageGC％ofthesequencewas

6、42％，andtheGCaveragedepthiSmoTethan40incoveragedistributionRepeatsequcnceisI0lGbaccountingfor3471％andtransposableelementsiSaccountingfor90％inrepeatsequenceUsingthegenestructureandgenefunctionannotation，21，704geneswerepred

7、icted，thefunctionof20，600geneswerenoted，thesizeofthenon—codingRNAaccountsfor0OI5％ofthegenomeTherearel7，015genefamiliesinMongoliansheepBasedonthegenomeresearch，weusedaCGHsystemtoidentifytheCNVsinthesheepgenomeandconstruct

8、edtheCNVsmapjnMongoliansheep，Kazakhsheep，Tibetansheep，HusheepandDorpersheep5lCNVRsweretestedcontaining24gains，22lossesand5gain—lossesTheHBBgenewasamplifiedinMongoliansheepandTibetansheepwhichmaybeattributedtoadaptability

9、inlowoxygenandhighaltitudeenvironmentForthecold—resistaneeofMongoliansheep，DoperSheepasreference，wesequencedthetranscriptomeoffat，muscles，adrenalglandsandhypothalamusinthecoldconditionusinglllumina／Solexatechnologyandsel

10、ected529，75，268andl61differentlyexpressedgenesAccordingtodifferentlyexpressedgenesof伍ttissuebyKEGGsignalingpathwaytheresultsweremainlyrelatedwithfatcelldifferentiation，lipidsynthesis，energymetabolismI33up—regulatedgenesw

11、erefilteredinfat，inwhichRXR，F(xiàn)ATP，AOP7involvedinPPARcellsignalingwiththefunctionofacceleratingpreadipocytedifferentiationandtransportinglong—chainfattyacid；GB氏T3ALDHinvolvedinglycerolipidmetabolismsignalingpathwayandregul

12、atethesynthesisoftriglyceride；1RSlandLEPinvolvedintheregulationofenergymetabolism；theribosome28Ssubunitandl8Ssubunitinvolvedinproteins審nthesis10differentlyexpressedgeneswhichplayedanimportantroleinlipidsynthesisandenergy

13、metabolismwereselectedtobeverifiedusingqPCRTheresultswereequivalenttORNA—seqIncoldconditions，theleveloflipidsynthesisandenergymetabolismandproteinsynthesisinfattissueofMongoliansheepwashigherthanDoperSheep，whichshowedMon