利用等位酶位點分析東鄉(xiāng)野生稻的遺傳多樣性.pdf

1、江西農(nóng)業(yè)大學(xué)碩士學(xué)位論文利用等位酶位點分析東鄉(xiāng)野生稻的遺傳多樣性姓名：李桂花申請學(xué)位級別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：黃英金劉宜柏2002.6.1UsingA||ozymatiClOCitoStudytheGenetiCDivorsityofDongxiangWildRiCe(OryzarufipoEonGriff)Abstract：ThenorthemmarginalpopulationofthecommonwildriceOru

2、fipogonGriffwasfoundinDongxiangCounty(28。147)ItwasreportedthatthispopulationpossessestheuniquecharacteristicstOricebreeders，suchastheabilitytotolerateextremecoldanddroughtandresisttomanykindsofpests，aswellashi曲proteincon

3、tentofthegrainsThispopulationisanimportantcomponentofthericegenepoolbecauseofitsgreatsignificanceinfindingusefulgenesforricegeneticimprovementandexploringtheoriginofAsiancultivatedriceHoweverthepopulationhasbeenseriously

4、threatenedandliesattheedgeofextinctionTherefore，insituconservationhadbeentakenfortwoexistingsubpopulationssince1992Inthepastdecades，ithasbeenstudiedbyJiangxiAcademyofAgriculturalSciencesInrecentyears，primaryexplanationso

5、fferedforendangermentarelossofhabitat，impactofnativeanimalsandplants，lossofassociatedspecieswithwhichtheaffectedplantshaveanobligaterelationshipButatthesametime，aspecies，inasense，isauniquegenepool，themorediverseandvariou

6、stheintro—specificgenesare，thebetterthespecieswilladapttOtheenvironments，andthegreaterpotentialthespecieswillhaveinitsevolutionInthisthesis，subpopulationgeneticsoftheDongxiangwildrice(OryzarufipogonGriff)weredetectedbyve

7、rticalslabpolyacrylamidegelelectrophoresisTwelveenzymesencodedbytwenty—onelociwasassessedfromninesubpopulationsThemainresultswereasfollows：1MediumandaslighthighlevelsofgeneticdiversitywereobtainedinDongxiangwildrice(Oryz

8、arufipogonGriff)Thepercentageofpolymorphicloci(P)，meannumberofallelesperlocus(A)，meanobservedheterozygosity(Ho)andmeanexpectedheterozygosity(He)were28O％，1302，0199and0223respectively2Thediversitywithinsubpopulation(Hs)was

9、0113whileamongdifferentsublcIopulations(Dst)wasO002，andthetotaldiversity(Ht)was0116，thecoefficientofgenedifferentiation(Gst)wasO015Thegeneticdiversityamongsubpopulations(15％)waslowerthanotherspeciesincommonwildrice(Oryza

10、rufipogonGaff)andmostvariation(985％、werewithinsubpopulations3Thehighsimilargeneticdiversity(O993～1000)showedthatallthesubpopulationcomesfromonepopulationTheresultssuggestedstrategiesfortheconservationofDongxiangwildriceK