鐵載體高產(chǎn)菌的篩選及鐵載體的分離純化.pdf

1、鐵載體高產(chǎn)菌的篩選及鐵載體的分離純化學(xué)位論文完成日期：2013—4—8指導(dǎo)教師簽字：莖蘭壘喳答辯委員會(huì)成員簽字：二蓋—吐泓J碰THESELECTlONOFHIGH—YIELDSIDEROPHPRESPRODUCINGSTRAINSANDSEPARATlONANDPURIFlCATIONOFTHESIDEROPHORESABSTRACTIronisanessentialelementofthelivingorganisms，itplays

2、animportantroleinthemetabolicprocessesoftheorganismsInmicrobialmetabolicprocesses，theironisthecofactororactivatorofmanyenzymes，suchascytochrome，cytochromeoxidase，catalase，ferredoxinprotein，ironsulfurproteaseandSOonOnthec

3、onditionthatorganismsarelackofiron，themetabolicprocessesabovewillbeimpactedsignificantlySiderophoreisalowmolecularweight(500—1000Oa)organiccompoundthatmicroorganismproducesinlowironstressconditions，andcancombinewithFe’ef

4、ficientlyItsbiosynthesisisstrictlycontrolledbytheconcentrationofironInthisstudy326strainsbacteriawereisolatedfromtheintestineandgillsofmarinefishesfromtheoffshoreareaofQingdaobyusingLBmediumand2216Emedium52high—yieldside

5、rophore—producingstrainswereselectedbyemployingCASsolidandliquidmethods，theirproductivityareallabove50％30ofthe52highyieldsiderophoreproducingstrainsCanproducehydroxamatesiderophores，16ofthoseCanproducecatecholsiderophore

6、sand8ofthoseCanproducebothofthembyironperchloratetests，ironchloridetests，csakytestsandarowntestsTheresultsshowthatthestrainCB—EH一2isthepredominantstrainofproducingsiderophoresSiderophoresproductionwas83116％andreached6299

7、mg／LStrainCBEH2wasidentificedasCobetiamarinausing16SrDNAbasedmethodDifferentconcentrationsofFe2十a(chǎn)nd22’bipyridinewererespectivelyaddedintothemediumforexploringtheireffectonsiderophoreproductionTheresultshowsthatwhenthe2，2

8、’bipyridineconcentrationreachs509mol／L，thesiderophoreproductionishighest；whenconcentrationof22’bipyridineexceeds200pmol／L，thestraindoesn’tgrowTheresultsshowsthattraceamountsof22’bipyridineCanstimulateCB—EH一2toproduceside