卵黃高磷蛋白及其磷酸肽的制備和研究.pdf

1、西北大學(xué)碩士學(xué)位論文卵黃高磷蛋白及其磷酸肽的制備和研究姓名：劉小麗申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化工指導(dǎo)教師：張小燕20030601ProduceandStudyonthePhosVitinandItsPhosphopeptidesAbstractnlenewtechnoIogy、ⅣasputfonvordtoextractphoSvitin(PV)舶meggy01kbasedont11emethodsofLossoJNa11dNaika

2、iSThetechnology、惴simple謝tllnootheraccretion，a11d也eo山ercomponentsofeggyolkwerecOnsideredtOa11mundusingTheprocessconditionsofhydrolysisofphosVitinby似ot)，pesofprote01”icer珂mewerestlldiedTheperf0咖aJlceoftheproductiontopreVen

3、tC一丘omd印ositionwassmdiedtooFormecomponemsofeggyoIk，watersoIubleproteinsexceptphosVitinwereremoved謝tllwaterfirStlytllentlleIipidswereeliminated、)l，i也organics01Vent，atlastⅡlcphosvitinw懿isolated丘Dmthereslllted口aIluleswithNa

4、Clsolution1hroughexp翻mentst11eoptimalprocessingconditionswereobtained嬲follows：t11edosage0fwaterand也eO唱aIlicsolvemwere100mL肌d20mLrespectiVely’theratiO0fetllOnalandnhexaIlew髂l：l(V，v)theNaClsolutionconcen仃砒ionw髂175mol／L111e

5、techllicalconditionsforhydr01ysisofphosVitinby2709alkalescenceandASl398ne曲面prote01yticenzymewerestIldiedTheresultshowedth砒t11edegreeofhydml”iswashighwhenphosvitinwashydr01ysisedaSf01lows：phosVitiIlwasdealed謝thNaOHfor15mi

6、nutefirstly’tllenhydr01)，zedbyalkalescenceproteol”icem可me2709for150minutesa11dhydrolyzedbyneutralproteolyticem可meASl398for60minutesat50ce甜筍adewhentllesubs仃ateconcennation、張s2％Theoptim啪pHvaluea11dconcennationforalkalescen

7、ceproteolyticenzyme2709、^raSpHlland5％，forneutra王proteol”icenzymeASl398waSpH75and6％respectiVelyTheproductionofhydrolyzingphosvitinwasphosVitinphosphopeptides(PPP)TheperfbnnanceofppptopreVentCr行omdeposition、^，ass1：udiedand

8、compared淅tllcaseinphoSphopeptides(cpp)neresu】tshowedtllatPPPcouldpreVentC∥丘omdepositionatace蹦nde冒ee鋤dmee髓ctw觴betterLiuXiaoli(ChemicalBiologyenginee“ngDirectedbyPrOfessOrZhangXiaoyanKeywords：phosvitinphosvitinphosphop印tid