ASICs阻斷劑對大鼠腦I-R損傷保護及其作用機制的研究.pdf

1、新疆醫(yī)科大學(xué)碩士學(xué)位論文ASICs阻斷劑對大鼠腦IR損傷保護及其作用機制的研究姓名：展群嶺申請學(xué)位級別：碩士專業(yè)：神經(jīng)內(nèi)科學(xué)指導(dǎo)教師：張小寧20061001新疆醫(yī)科大學(xué)醫(yī)學(xué)碩士學(xué)位論文新疆醫(yī)科大學(xué)醫(yī)學(xué)碩士學(xué)位論文２TheStudyofNeuroprotectiveEffectMechanismofASICsBlockeronIschemiaReperfusionInjuryinRatBrainGraduate:ZhanQunlingTu

2、t:ZhangXiaoningTheFirstClinicalMedicalCollegeXinjiangMedicalUniversityABSTRACTObjective:TheaimofthisstudywastoinvestigatetheneuroprotectiveeffectmechanismofASICsblockeronischemiareperfusioninjuryinratbrain.Methods:Themod

3、eloffocalcerebralischemiareperfusioninSpragueDawley(SD)ratswasestablishedwithsuturedoccludedmethodinventedbyZeaLonga.SixtyfourSDratswereromlydividedintofourgroupsincludingshamoperatedgroupfocalcerebralischemiareperfusion

4、modelgroupamilideⅠgroup(repetitiveadministrationtwotimes)amilideⅡgroup(repetitiveadministrationfivetimes)eachgroupwassixteenrats.Firsttheeightratsineachgroupwereromlytakenftheevaluationofneurologicaldeficitscethedetermin

5、ationtocerebralinfarctvolumeHEstainofbraintissueattwentyfourhoursreperfusionaftertwohoursfocalbrainischemia.Secondtheothereightratsineachgroupweretakenfdeterminationtocontentsofmyeloperoxidase(MPO)nitricoxide(NO)fromtheb

6、raintissuehomogenatebybiochemicalreagentsattwentyfourhoursreperfusionaftertwohoursfocalbrainischemiaunderthesamemodel.Results:(1)ComparedwithamilideⅠamilideⅡgroupthesceofneurologicaldeficitbraininfarctvolumeinthefocalcer