pcdna3.1mychisb()tpa質(zhì)粒dna交聯(lián)明膠微球復(fù)合物dacron片局部轉(zhuǎn)染大鼠骨骼肌的實(shí)驗(yàn)研究

1、華中科技大學(xué)碩士學(xué)位論文pcDNA3.1-Myc-HisB（-）/tPA質(zhì)粒DNA-交聯(lián)明膠微球復(fù)合物Dacron片局部轉(zhuǎn)染大鼠骨骼肌的實(shí)驗(yàn)研究姓名：李師亮申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：心血管外科指導(dǎo)教師：張凱倫20090501華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文 4Experimental Research of Local Transfection of Dacron patch w

2、ith pcDNA3.1-Myc-His B(-)/tPA plasmid DNA-Cross Linking Gelatin Microspheres complex into rat muscle Abstract Objective: To detect local gene transfection efficiency and expression of tPA protein after gene transfection

3、of pcDNA3.1-Myc-HisB(-)/tPA plasmid DNA being transferred into rat skeletal muscle, which was released from Dacron patch with pcDNA3.1-Myc-His B(-)/tPA plasmid DNA-Cross Linking Gelatin Microspheres complex in vivo. Meth

4、od: Thirty male Sprague-Dawley rats were randomly divided into 3 groups (weight,50-100g);(1)pcDNA3.1-Myc-His B(-)/tPA Plasmid DNA-Cross Linking Gelatin Microspheres Complex(n=10).(2)pcDNA3.1-Myc-His B(-)/tPA Plasmid DNA

5、solution (n=10). (3) pcDNA3.1 Plasmid DNA-Cross Linking Gelatin Microspheres Complex(n=10). Target gene was locally delivered into gastrocnemius muscles. Samples were collected at different time .Real-time PCR was used t

6、o detect tPA-mRNA and Western blotting was used to detect tPA protein synthesized in gastrocnemius muscles after transfection. Results:The transcription and the protein expression of Exogenous tPAmRNA was detected in bot

7、h Experimental group and control group at 1,3,5,7,14,28 ,56days after operation . Conclusion: pcDNA3.1-Myc-HisB(-)/tPA plasmid DNA which was released from Dacron patch with pcDNA3.1-Myc-His B(-)/tPA plasmid DNA-Cross Lin

8、king Gelatin Microspheres complex could be successfully locally transfected into rat skeletal muscle and tPA protein could be successfully expressed. Key Word: pcDNA3.1-Myc-HisB(-)/tPA plasmid DNA; Cross Linking Gelatin