細胞凋亡影響CIK細胞抗腫瘤功能的作用及其調控機制.pdf

1、廣東藥科大學碩士學位論文分類號：分類號：___________密級密級:UDC：___________細胞凋亡影響CIK細胞抗腫瘤功能的作用及其調控機制TheeffectregulationmechanismofCIKapoptosisontheantitumactivity姓名：劉愛梅學號：2111340051院系：藥科學院專業(yè)：微生物與生化藥學研究方向：生物技術藥物導師姓名：___孫艷（副教授）論文提交日期：2016年3月19日論文

2、答辯日期：2016年5月23日學位授予單位：廣東藥科大學二○一六年五月廣東藥科大學碩士學位論文I目錄目錄目錄..........................................................................................................................................I摘要.........................

3、..............................................................................................................IVAbstract....................................................................................................

4、..............................V前言.........................................................................................................................................1第一部分不同來源的CIK細胞誘導.................................

5、...............................................101材料與方法.......................................................................................................................101.1實驗樣本.....................................

6、.............................................................................101.2主要儀器.................................................................................................................111.3主要試劑............

7、.....................................................................................................111.4主要溶液配制.........................................................................................................121

8、.5實驗方法與步驟.....................................................................................................121.5.1CIK細胞的分離培養(yǎng)....................................................................................121.5.2細胞

9、計數(shù).......................................................................................................131.5.3流式細胞術檢測不同來源的CIK細胞表面標記的表達...........................131.5.4統(tǒng)計學方法.............................................

10、......................................................132結果....................................................................................................................................142.1形態(tài)學觀察不同來源的CIK細胞.........

11、................................................................142.2不同來源的CIK細胞生長情況............................................................................152.3CBCIK細胞誘導過程中細胞亞群變化......................................

12、........................152.5不同來源CIK細胞亞群比較................................................................................183結論.............................................................................................

13、......................................194討論...................................................................................................................................20第二部分CIK細胞凋亡對其體外殺傷腫瘤功能的影響....................

14、.............................221材料與方法........................................................................................................................221.1實驗樣本......................................................

15、............................................................221.2主要儀器.................................................................................................................231.3主要試劑.............................