紫外線對(duì)細(xì)胞DNA損傷與損傷中鈣離子信號(hào)變化的熒光檢測(cè)分析.pdf

1、Y79G‘89分類號(hào)————密級(jí)——西北大學(xué)單位代碼：一1061L學(xué)號(hào)：!Q!!墊!!碩士學(xué)位論文題目鱉鹽垡壁塑墮塑壘塑叢生塑笪主塹蠱孟焦量塞垡盟墓坐拴型金盤作者鱟垃指導(dǎo)教師堡塑專業(yè)技術(shù)職務(wù)盟窒亟指導(dǎo)教師堡!墾至!壺造專業(yè)技術(shù)職務(wù)塾蕉學(xué)科(專業(yè))墨鱟——答辯日期20056學(xué)位授予日期——二00五年五月AbstractThroughexaminingtheDNAdamageandtheCa”densityofrathepatocyte，w

2、hichwasirradiatedbythreekindsofdifferentwavebandsofUVtherelationbetweentheDNAdamagelevelandtheirradiativedosageofUVwasdetermined[Ca2】iduringthedamagewasdetectedThenthemechanismofUVdamageandrepairwasrevealedBesidesthetype

3、sandthereasonsoftheDNAdamage011biologycell，thevariousmethodsusedtodeterminethedamagewereintroduced，andthemechanismofCa2signalsactionintheprocessoftheDNAdamagewasanalyzedUsingsingle—cellgelelectrophoresis，theDNAdamagewass

4、tudiedwhenthesmallratlivercellwasirradiatedbydifferentwavebandsanddifferentdosageofUV[Ca2】iwastestedbyratiofluorescenceimagingsystemAnalyzingthedataofthetests，thegraphofrelationshipbetweenthedegreeofDNAdamageandthequanti

5、tyofdifferentUV’SwavelengthwasdrawnOUtTheresultswereshownasfollows：(1)WhethertheUVcandamagetheDNAliesontheUV’Swavelength(orfrequency)；(2)TheirradiatedenergyofUVisindirectproportiontOthedegreeoftheDNAdamage；(3)Thereisthef

6、eatureofhighenergyflatandlowenergythresholdvalueinthegraph；(4)[Ca2】iwillriseduringthedamageTheamplitudeandthefrequencywillvarywithtimeKeywordUV；Photoelectriceffect；RadiationenergythresholdvalueRathepatocyte；Singlecellgel