角類肥蛛抗菌物質的誘導、活性測定及紫外線照射對蜘蛛DNA的損傷研究.pdf

1、湖北大學碩士學位論文角類肥蛛抗菌物質的誘導、活性測定及紫外線照射對蜘蛛DNA的損傷研究姓名：徐慧君申請學位級別：碩士專業(yè)：生態(tài)學指導教師：彭宇20080501AbstractPhysicalandchemicalstimulationCallbeinducedspiderstoobtainantibacterialsubstancesInthispaper，Antibacterialmaterialwereextractedfromth

2、eadultsofLarinioidescornutawhichwereinducedbyUVtheproteinandcellulardebriswereremovedfromtheextractionbycentrifuging，thenstudiedthestabilityofantibacterialmaterialOnthisbasis，wedetectedthelevelofthespider’SDNAdamageinUVi

3、nductionprocessbysinglecellgelelectrophoresisTheresultsareasfollows：Lcornutawereinduced12，3，4，5，6，7，8，9，10，11，12，24，36，48hoursrespectivelybyUVAfterkeepingwiththefruitflyandwaterfor60h，extractingtheantibacterialsubstances

4、anddetectingtheantibacterialactivityWefoundthatantibacterialmaterialswerestabilityindifferentinducestagesIntheexperimentoftheheatresistantcapacityoftheantibacterialsubstancesbyLiquidcultureinhibitionassayTherusultsshowed

5、thattheantibacterialsubstancesoftheinducedLcornutawithacertainheatstabilitythehighesttemperatureheatresistantcapacityis80℃handle15minandinthiscircumstancesantibacterialsubstanceswithminimalactivityTheantibacterialsubstan

6、ceshaveantibacterialactivityafter10timesfreezethawalternateatroomtemperatureTheresultdetectthatthe厶cornutaantibactedalsubstanceshavestrongantifreezethawcapacityExtractionsofantibacterialsubstancesbetweenpH3topHllhaveacti

7、vityBetweanpH4topill0theantibacterialactivitywasmorestableButtheactivitywassignificantlylowerinpH10Theresultshowedthatthe厶cornuta’SantibacterialsubstanceshasstrongacidalkalicapacityTheantimicrobialsubstancesextractionkee

8、pedantibacterialstabilityin0056mol／LacidammoniumsaltconcentrationTheantibacterialactivityhadacertaineffectbysaltconcentration，buttheimpactisnotsignificantdifferencesAfter24hculturewithantibacterialsubstance，Heladidn’tgro