酵母菌產(chǎn)類金屬硫蛋白的分離純化、鑒定及培養(yǎng)條件優(yōu)化.pdf

1、南開大學(xué)碩士學(xué)位論文酵母菌產(chǎn)類金屬硫蛋白的分離純化、鑒定及培養(yǎng)條件優(yōu)化姓名：李登文申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：微生物指導(dǎo)教師：邢來(lái)君2000.5.1AbstractSaccharomycescerevisiaeCu一21wasselectedfromninestrainswhichcouldresistanttometalcopperTheselectedstraincouldresistanttoheavymentalandproduce

2、moreM棼lallo強(qiáng)ionel辯一like(MT—like)thanotherstrainsbyinducedwithcopperionsToobtainthebestculturecondition，thestrainwasscreenedandmeasuredwithseveraldifferentcultivateconditionswhichinvolvedactivationperiodoftime，inducingper

3、iodoftime，thekindandconcentrationofinducerSothebestcultivatedconditionwas：activationtime24h，inductiontime48handinducedby1mmol／LCuCl2whichwasessentialfor醚elallothio矗ei扣likeproductionTheproteinswerereleasedafterthewallsofy

4、eastwerebrokenTheseproteinswereisolatedandpurifiedbySephadexG”100andsubsequentDEAE52，thendialysisfordesalinationThentherewerethreeiso—M瓠forcopperinducingThemolecularweightswereabout7kDTheisoelectricpointwasabout62Thecont

5、entofCysteinewashighamongaminoacidcompositionandupto2025％EachmolecularofMTscombined7gcopperions，Theisolatedproteinshadspecialabsorbencyspectrums，whichworecreatedbymetalatomsbindingtotheproteinsThepurifiedproteinswereMTsl