基于響應(yīng)界面技術(shù)蓖麻餅粕脫毒工藝及高速逆流色譜純化分離水脫毒液中蓖麻堿.pdf

1、基于響應(yīng)界面技術(shù)蓖麻餅粕脫毒工藝及高速逆流色譜純化分離水脫毒液中蓖麻堿StudyonDetoxificationofCastCakeBasedonResponseInterfaceTechnologyHighSpeedCounterCurrentChromatographyPurificationofRicinlinePurificationProcessinDetoxificationSolution申請人：周佳儒學(xué)科專業(yè)：有機(jī)化學(xué)研

2、究方向：天然產(chǎn)物化學(xué)學(xué)位類別：學(xué)術(shù)學(xué)位指導(dǎo)教師：李久明教授提交日期：二零一八年六月分類號(hào)ICS學(xué)校代碼10136學(xué)號(hào)201500221碩士學(xué)位論文StudyonDetoxificationofCastCakeBasedonResponseInterfaceTechnologyHighSpeedCounterCurrentChromatographyPurificationofRicinlinePurificationProcessinD

3、etoxificationSolutionAbstractCastcakeistheresidueaftertheoilisextractedfromcastbaen.Itcontainsproteinsthatcanbeabsbedbyanimals.Howeverduetocontainingtoxicsubstancessuchasrieinineallergenswhichcannotbedirectlyusedinthefee

4、d.ThispaperestablishesarieininecontentdetectionmethodwithHPLC.Usingmicrowavemethodultrasonicmethodwatervapmethodtodetoxifycastcaketheremovalindertemoverieinine.AtthesametimeusingHighSpeedCountercurrentChromatographysepar

5、ationofrieininebycomparingthedifferentexperimentalconditionsindertotogetthemostoptimumchromatographicconditions.Theresultsareasfollows:AHPLCmethodwasestablishedfthedeterminationofricin.Inthisexperimenttheexperimentwascon

6、ductedbycomparingdifferentchromatographiccolumnsdetectionwavelengthsdifferentflowphases.ChoosingthechromatographiccolumnXAeC185μm4.6250mmDetectionwavelengthat310nmGradientelutionusingmixedsolutionsofwateracetonitrileindi

7、fferentproptionsFlowrateof1.00mL?min1Columntemperatureof20℃Samplequantityof20μLasthetestconditionssettingupaneffectiverapiddetectionmethodthentestingthreebatchesofrieininecontentinthecastbeancake.IntheendTherelativestard

8、deviationsofthemeasuredcontentswere0.161%0.410%1.17%.Optimizationofoptimumdetoxificationprocessofmicrowavemethodultrasonicmethod.OnthebasisofmicrowavesinglefactexperimentaccdingtotheprincipleofBoxBehnkencentralcomposited

9、esigncombinationexperimentaldesigncenterresult.Microwavedetoxificationconditions:microwavepoweris500Wmicrowavetimeto36ssolidliquidratioof1:9microwavefrequencyf5times.Inthisexperimenttheremovalrateofrieininewas79.102%rici

10、nineresiduecontent0.0655%Ultrasonicdetoxificationconditions:thesolidliquidratiowas1:20theultrasonictimewas50mintheultrasonicfrequencywas4times.Inthisexperimenttheremovalrateofrieininewas94.21%ricinineresiduecontent0.0181