喜樹中主要生物堿提取分離工藝的研究.pdf

1、UniversityCode：10225RegisterCode：09663DissertationfortheDegreeofMasterSTUDYONEXTRACTIONANDPROCESS0FMAINALKALOIDSⅢ例御m腳例么C硼釷M尉Candidate：Supervisor：AcademicDegreeAppliedfor：Speciality：DateofOralExamination：University：LIJiah

2、uiAssociateProfZhaoChunjianMasterofMedicalPharmcognosyJune，2009NortheastForestryUniversityAbstractThisstudytookCamptothecaacuminata(Nyssaceae)asmaterial，anindigenousspeciesofChina，alsopilotstudyondistributionofCamptothec

3、inand10一hydroxycamptothecininitBasedonthestudymentionedabove，extractionfromCarnptothecin(CPT)and10hydrxycamptothecin(HCPT)inseedsandleaveSofCamptothecaacuminatawereoptimizedbyresponsesurfacemethodology(RSM)Optimumtechnol

4、ogyparametersonseedsthat50％ofethanolvolumefraction，3rainofhomogenatetimeand7(mL：g)ofliquortomaterial，while，optimumtechnologyparamersonleavesthat70％ofethanolvolumefraction，6rainofhomogenatetimeand1l(mL：g)ofliquortomateria

5、lExploringpurificationprocessofCamptothecinasorganicsolventextraction，liquid—liquidextraction，recrystallizationetc，anddeterminedtheOptimalconditionsoftheexperiments，finallyinpilotmagnification，thepurityofCPTinextracumis9

6、8％andtheextractionyieldofcptis00216％OptimizedseparatingmethodofHCPTfromtheremainderextractsfortheproductionofCPTwithmacroporousresinsadsorption—desorptionX一5resinoffersthebestadsorptionanddesorptioncapacitiesforHCPTandth

7、eoptimumconditionsforadsorptionwerethatpHwas5，40℃oftemperature，4BV／hofflowrateand5BVofsampleprocessingvolume。TheoptimumconditionsfordesorptionwerethatlO一90％ofethanolwereusedassolventsforgradientelution，30—60％ofethanolelu

8、ateswerecollectedasfinalproductwhichwasconcentratedTheflowratewas4BV／handthevolumeofelutionwas2BVThemid—testresultsshowthatthecontentofHCPTintheproductincreasedtO719％。andtherecoveryyieldwas821％Optimizationofrecrystalliza

9、tiontopurifiedHCPT，anditspurityachieved95％。ThisthesisisobtainedformaterialsonseedsandleavesofCamptothecaacuminata，atthesametimeproducingCPTandHCPTonmid—testThistechnologyislowerproductioncosts，higherratesandhighlypure，an