番茄多聚半乳糖醛酸酶（PG）反義基因表達(dá)及相關(guān)功能研究.pdf

1、中國(guó)農(nóng)業(yè)大學(xué)博士學(xué)位論文番茄多聚半乳糖醛酸酶（PG）反義基因表達(dá)及相關(guān)功能研究姓名：寇曉虹申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：食品科學(xué)指導(dǎo)教師：羅云波20030501AbstractPolygalacturonase(PG)playsmajorrolesinfruitsoftening，organabscission，pollinationandseeddevelopmentTherelationshipbetweenpolygalacturona

2、seandethylene，otherplanthormone，andthepositionsofPGinplantresistanceanddiseasesresponseswerespeciallydiscussedinthispaperThemultiformityofPGfunctionswasindicatedAgrobacteriummediatedtransformationhadbeenusedtointroduceth

3、eAntiPGgeneintoprocessingtomato(cv98—75)FourpositivetransformedtomatoplantswereconfirmedbyPCRandSouthernhybridizationFruitfirmnessandsolublesolidoftransgenictomatowerehigherthanthoseofcontrol；fruitwerefullandlustrous；sol

4、ublepectinwaslowerthancontrolatthesamestageThePGactivityoftransgenictomatowas83％，88％，63％，46％ofcontroltomatespectivelyNorthernhybridizationshowedthatallPG，LOgandLeEXPlmRNAdecreasedintransgenictomatofruitcomparedwiththewil

5、dtomatoTheseresultssuggestthattheprocessofripeningandsofteningintransgenicantiPGtomatoweredelayed，TheexpressionpatternsofPGLeEXPlandLOXwerestudiedinwildtomato，antisenseACStomatoandNrmutanttomatoTheresultshowedthatPGplaye

6、damajorroleinripeningandsofteningoftomatofruitandcooperatedwiththeregulationofLeEXPlandLOXTherelationshipbetweenathyleneandPolygalacturonase儼(甜WasthoroughlyinvestigatedintwoaspectsoftheeffectofethyleneonPGfunctionandther

7、egulationofPGonethylenesynthesisWithwildtomato，antisenseACStomato，Nrmutanttomatoandwildtomatotreatedwithexogenousethyleneand1MCPasmaterials，itwasshowedthattheexpressionofPGgeneandPGactivitywereinhibitedsignificantlyinant

8、isenseACStomatoandNrmutanttomatoTheexpressionofPGwaspromotedwithexogenousethylene(100p1／L)andobviouslyinhibitedwithl—MCP(1I_tl／L)TheseresultssuggestedthatexpressionofPGgenewasregulatedbyethyleneOntheotherhand，withantisen

9、sePGtomatoandwildtomatotreatedwith100mg／LDGAasmaterials，itwasshowedthattheethyleneproductionofantisensePGtomatowaslowerthanthatofwildtomato，theexpressionofethylenereceptorgeneLeETR4andtheethyleneresponsefactorLeERF2werei

10、nhibitedTheethylenesynthesiswasaffectedbychangesofexpressionmodelonPGgeneTheethyleneproductionandexpressionofLeETR4andLeERF2werepromotedwiththetreatmentof100mg／LD—GATheseresultsimplicatedthatthesynthesisandactionofethyle

11、newerealsofeedbackregulatedbyPGgeneinsomecaseThereisaninteractionbetweenethyleneandPGWildtomatoantisenseACStomatoandNrmutanttomatoweretreatedwith01mMABA，0ImMGA，0ImMIAA，01mM6BAand1mMSATheresultssuggestedthattheexpressiono